The Tandem Repeat AGGGTAGGGT Is, in the Fission Yeast, a Proximal Activation Sequence and Activates Basal Transcription Mediated by the Sequence TGTGACTG

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1995 Nov 11

PMID 7501449

Citations 14

Authors

I Witt

M Kwart

T Gross

N F Kaufer

Affiliations

Soon will be listed here.

Abstract

Ribosomal protein (rp) genes in the fission yeast Schizosaccharomyces pombe display two highly conserved sequence elements in the promoter region. The molecular dissection of these promoters revealed that basal transcription is not based on a TATA element. The sequence which promotes basal transcription is the conserved sequence CAGTCACA or the inverted form TGTGACTG, called the homol D box. Upstream of the homol D box a tandem repeat AGGGTAGGGT or the inverted form ACCCTACCCT appears in some promoters, called homol E. This element functions in the proximal arrangement with homol D as an activation sequence. A compilation of homol D and homol E sequences identified in other S.pombe promoters revealed that several putative polymerase II and polymerase III promoters display a homol D box or the homol E/homol D arrangement.

Citing Articles

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References

Kaufer N, Fried H, Schwindinger W, Jasin M, Warner J . Cycloheximide resistance in yeast: the gene and its protein. Nucleic Acids Res. 1983; 11(10):3123-35. PMC: 325953. DOI: 10.1093/nar/11.10.3123. View

Harrington R, Winicov I . New concepts in protein-DNA recognition: sequence-directed DNA bending and flexibility. Prog Nucleic Acid Res Mol Biol. 1994; 47:195-270. DOI: 10.1016/s0079-6603(08)60253-6. View

Russell P . Transcription of the triose-phosphate-isomerase gene of Schizosaccharomyces pombe initiates from a start point different from that in Saccharomyces cerevisiae. Gene. 1985; 40(1):125-30. DOI: 10.1016/0378-1119(85)90031-9. View

Nischt R, Gross T, Gatermann K, Swida U, Kaufer N . Sequence and regulatory responses of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res. 1987; 15(4):1477-92. PMC: 340562. DOI: 10.1093/nar/15.4.1477. View

Gross T, Nischt R, Gatermann K, Swida U, Kaufer N . Primary structure of the ribosomal protein gene S6 from Schizosaccharomyces pombe. Curr Genet. 1988; 13(1):57-63. DOI: 10.1007/BF00365757. View

PORTER G, Brennwald P, Holm K, Wise J . The sequence of U3 from Schizosaccharomyces pombe suggests structural divergence of this snRNA between metazoans and unicellular eukaryotes. Nucleic Acids Res. 1988; 16(21):10131-52. PMC: 338842. DOI: 10.1093/nar/16.21.10131. View

Fleming G, Belhumeur P, Skup D, Fried H . Functional substitution of mouse ribosomal protein L27' for yeast ribosomal protein L29 in yeast ribosomes. Proc Natl Acad Sci U S A. 1989; 86(1):217-21. PMC: 286435. DOI: 10.1073/pnas.86.1.217. View

Grimm C, Kohli J, Murray J, Maundrell K . Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet. 1988; 215(1):81-6. DOI: 10.1007/BF00331307. View

Dandekar T, Ribes V, Tollervey D . Schizosaccharomyces pombe U4 small nuclear RNA closely resembles vertebrate U4 and is required for growth. J Mol Biol. 1989; 208(3):371-9. DOI: 10.1016/0022-2836(89)90502-0. View

10.

Teletski C, Kaufer N . Sequence of the ribosomal protein gene KD4 from Schizosaccharomyces pombe. Nucleic Acids Res. 1989; 17(23):10118. PMC: 335254. DOI: 10.1093/nar/17.23.10118. View

11.

Gatermann K, Teletski C, Gross T, Kaufer N . A ribosomal protein gene family from Schizosaccharomyces pombe consisting of three active members. Curr Genet. 1989; 16(5-6):361-7. DOI: 10.1007/BF00340715. View

12.

Beltrame M, Bianchi M . A gene family for acidic ribosomal proteins in Schizosaccharomyces pombe: two essential and two nonessential genes. Mol Cell Biol. 1990; 10(5):2341-8. PMC: 360582. DOI: 10.1128/mcb.10.5.2341-2348.1990. View

13.

Porter G, Brennwald P, Wise J . U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene. Mol Cell Biol. 1990; 10(6):2874-81. PMC: 360649. DOI: 10.1128/mcb.10.6.2874-2881.1990. View

14.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

15.

Moreno S, Klar A, Nurse P . Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 1991; 194:795-823. DOI: 10.1016/0076-6879(91)94059-l. View

16.

Damagnez V, DE RECONDO A, Baldacci G . Identification of a gene encoding the predicted ribosomal protein L7b divergently transcribed from POL1 in fission yeast Schizosaccharomyces pombe. Nucleic Acids Res. 1991; 19(5):1099-104. PMC: 333787. DOI: 10.1093/nar/19.5.1099. View

17.

Selinger D, PORTER G, Brennwald P, Wise J . The two similarly expressed genes encoding U3 snRNA in Schizosaccharomyces pombe lack introns. Mol Biol Evol. 1992; 9(2):297-308. DOI: 10.1093/oxfordjournals.molbev.a040721. View

18.

Marks J, Simanis V . Cloning of the gene for ribosomal protein S13 from the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res. 1992; 20(15):4094. PMC: 334097. DOI: 10.1093/nar/20.15.4094. View

19.

Witt I, Straub N, Kaufer N, Gross T . The CAGTCACA box in the fission yeast Schizosaccharomyces pombe functions like a TATA element and binds a novel factor. EMBO J. 1993; 12(3):1201-8. PMC: 413323. DOI: 10.1002/j.1460-2075.1993.tb05761.x. View

20.

Girard J, Feliu J, Caizergues-Ferrer M, Lapeyre B . Study of multiple fibrillarin mRNAs reveals that 3' end formation in Schizosaccharomyces pombe is sensitive to cold shock. Nucleic Acids Res. 1993; 21(8):1881-7. PMC: 309428. DOI: 10.1093/nar/21.8.1881. View