Association of Reovirus Outer Capsid Proteins Sigma 3 and Mu 1 Causes a Conformational Change That Renders Sigma 3 Protease Sensitive

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1995 Dec 1

PMID 7494347

Citations 17

Authors

D A Shepard

J G Ehnstrom

L A Schiff

Affiliations

Soon will be listed here.

Abstract

Association of the reovirus proteins sigma 3 and mu 1 influences viral entry, initiation of outer capsid assembly, and modulation of the effect of sigma 3 on cellular translation. In this study, we have addressed whether structural changes occur in sigma 3 as a result of its interaction with mu 1. Using differences in protease sensitivity to detect conformationally distinct forms of sigma 3, we showed that association of sigma 3 with mu 1 caused a conformational change in sigma 3 that converted it from a protease-resistant to a protease-sensitive structure and occurred posttranslationally. The effect of mu 1 on the structure of sigma 3 was stoichiometric. Our results are consistent with a model in which sigma 3's association with mu 1 shifts its function from translational control to assembly of an outer capsid in which sigma 3 is folded into the protease-sensitive conformation that is required for its cleavage during the next round of infection.

Citing Articles

Strain-specific differences in reovirus infection of murine macrophages segregate with polymorphisms in viral outer-capsid protein σ3.

Fiske K, Brigleb P, Sanchez L, Hinterleitner R, Taylor G, Dermody T J Virol. 2024; 98(11):e0114724.

PMID: 39431846 PMC: 11575339. DOI: 10.1128/jvi.01147-24.

Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly.

Kniert J, Dos Santos T, Eaton H, Cho W, Plummer G, Shmulevitz M PLoS Pathog. 2022; 18(9):e1010641.

PMID: 36099325 PMC: 9514668. DOI: 10.1371/journal.ppat.1010641.

Reovirus σ3 Protein Limits Interferon Expression and Cell Death Induction.

Roebke K, Guo Y, Parker J, Danthi P J Virol. 2020; 94(22).

PMID: 32847863 PMC: 7592210. DOI: 10.1128/JVI.01485-20.

Cell Entry-Independent Role for the Reovirus μ1 Protein in Regulating Necroptosis and the Accumulation of Viral Gene Products.

Roebke K, Danthi P J Virol. 2019; 93(11).

PMID: 30894465 PMC: 6532083. DOI: 10.1128/JVI.00199-19.

Potential for Improving Potency and Specificity of Reovirus Oncolysis with Next-Generation Reovirus Variants.

Mohamed A, Johnston R, Shmulevitz M Viruses. 2015; 7(12):6251-78.

PMID: 26633466 PMC: 4690860. DOI: 10.3390/v7122936.

References

Smith R, Zweerink H, Joklik W . Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969; 39(4):791-810. DOI: 10.1016/0042-6822(69)90017-8. View

Kedl R, Schmechel S, Schiff L . Comparative sequence analysis of the reovirus S4 genes from 13 serotype 1 and serotype 3 field isolates. J Virol. 1995; 69(1):552-9. PMC: 188609. DOI: 10.1128/JVI.69.1.552-559.1995. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Fields B, Raine C, Baum S . Temperature-sensitive mutants of reovirus type 3: defects in viral maturation as studied by immunofluorescence and electron microscopy. Virology. 1971; 43(3):569-78. DOI: 10.1016/0042-6822(71)90282-0. View

Chang C, Zweerink H . Fate of parental reovirus in infected cell. Virology. 1971; 46(3):544-55. DOI: 10.1016/0042-6822(71)90058-4. View

Silverstein S, Astell C, LEVIN D, Schonberg M, Acs G . The mechanisms of reovirus uncoating and gene activation in vivo. Virology. 1972; 47(3):797-806. DOI: 10.1016/0042-6822(72)90571-5. View

Astell C, Silverstein S, LEVIN D, Acs G . Regulation of the reovirus RNA transcriptase by a viral capsomere protein. Virology. 1972; 48(3):648-54. DOI: 10.1016/0042-6822(72)90149-3. View

Joklik W . Studies on the effect of chymotrypsin on reovirions. Virology. 1972; 49(3):700-15. DOI: 10.1016/0042-6822(72)90527-2. View

Shatkin A, LaFiandra A . Transcription by infectious subviral particles of reovirus. J Virol. 1972; 10(4):698-706. PMC: 356523. DOI: 10.1128/JVI.10.4.698-706.1972. View

10.

Borsa J, Copps T, Sargent M, Long D, Chapman J . New intermediate subviral particles in the in vitro uncoating of reovirus virions by chymotrypsin. J Virol. 1973; 11(4):552-64. PMC: 355137. DOI: 10.1128/JVI.11.4.552-564.1973. View

11.

Morgan E, Zweerink H . Reovirus morphogenesis. Corelike particles in cells infected at 39 degrees with wild-type reovirus and temperature-sensitive mutants of groups B and G. Virology. 1974; 59(2):556-65. DOI: 10.1016/0042-6822(74)90465-6. View

12.

Huismans H, Joklik W . Reovirus-coded polypeptides in infected cells: isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectively. Virology. 1976; 70(2):411-24. DOI: 10.1016/0042-6822(76)90282-8. View

13.

Mustoe T, Ramig R, Sharpe A, Fields B . A genetic map of reovirus. III. Assignment of the double-stranded RNA-positive mutant groups A, B, and G to genome segments. Virology. 1978; 85(2):545-56. DOI: 10.1016/0042-6822(78)90460-9. View

14.

McCrae M, Joklik W . The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3. Virology. 1978; 89(2):578-93. DOI: 10.1016/0042-6822(78)90199-x. View

15.

Mustoe T, Ramig R, Sharpe A, Fields B . Genetics of reovirus: identification of the ds RNA segments encoding the polypeptides of the mu and sigma size classes. Virology. 1978; 89(2):594-604. DOI: 10.1016/0042-6822(78)90200-3. View

16.

Drayna D, Fields B . Activation and characterization of the reovirus transcriptase: genetic analysis. J Virol. 1982; 41(1):110-8. PMC: 256731. DOI: 10.1128/JVI.41.1.110-118.1982. View

17.

Sharpe A, Fields B . Reovirus inhibition of cellular RNA and protein synthesis: role of the S4 gene. Virology. 1982; 122(2):381-91. DOI: 10.1016/0042-6822(82)90237-9. View

18.

Drayna D, Fields B . Genetic studies on the mechanism of chemical and physical inactivation of reovirus. J Gen Virol. 1982; 63 (Pt 1):149-59. DOI: 10.1099/0022-1317-63-1-149. View

19.

Drayna D, Fields B . Biochemical studies on the mechanism of chemical and physical inactivation of reovirus. J Gen Virol. 1982; 63 (Pt 1):161-70. DOI: 10.1099/0022-1317-63-1-161. View

20.

McPhillips T, Ramig R . Extragenic suppression of temperature-sensitive phenotype in reovirus: mapping suppressor mutations. Virology. 1984; 135(2):428-39. DOI: 10.1016/0042-6822(84)90198-3. View