An Immunohistochemical Investigation of Porcine Epidemic Diarrhoea

Overview

Journal J Comp Pathol

Publisher Elsevier

Specialties Pathology
Veterinary Medicine

Date 1995 Jul 1

PMID 7490338

Citations 55

Authors

M Sueyoshi

T Tsuda

K Yamazaki

K Yoshida

M Nakazawa

K Sato

T Minami

K Iwashita

M Watanabe

Y Suzuki

Affiliations

Soon will be listed here.

Abstract

A sudden outbreak of epidemic diarrhoea of piglets occurred in Japan, the principal features being watery diarrhoea, dehydration and high mortality in newborn animals. The microscopical lesions were villous atrophy in the small intestine, the villous enterocytes being vacuolated and cuboidal in shape. The villus-crypt ratio was severely reduced, varying from 1:1 to 3:1. Transmission electron microscopy showed numerous coronaviruses within the cytoplasm of enterocytes and among microvilli. Specific antigens of porcine epidemic diarrhoea (PED) virus were detected in the cytoplasm of enterocytes by the streptavidin-biotin (SAB) technique. Infected cells, which were most abundant in the villous epithelia of the jejunum and ileum, were present in small numbers in the large intestine, the crypt epithelia, the lamina propria and Peyer's patches. The study suggests that the SAB technique is useful for the diagnosis of PED.

Citing Articles

Prevalence and genetic diversity of porcine epidemic diarrhea virus in Southwest China during 2020-2022.

Xu T, Zhou Y, Liu Z, Zhang J, Wu F, You D Sci Rep. 2024; 14(1):29124.

PMID: 39582049 PMC: 11586391. DOI: 10.1038/s41598-024-80844-x.

Development and characterization of segment-specific enteroids from the pig small intestine in Matrigel and transwell inserts: insights into susceptibility to porcine epidemic diarrhea Virus.

Yen L, Nelli R, Twu N, Mora-Diaz J, Castillo G, Sitthicharoenchai P Front Immunol. 2024; 15:1451154.

PMID: 39355235 PMC: 11442308. DOI: 10.3389/fimmu.2024.1451154.

Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines.

Wu J, Gao H, Rui H, Xu P, Ni L, Zhang J Virus Genes. 2024; 60(6):667-673.

PMID: 39312036 DOI: 10.1007/s11262-024-02109-z.

Investigation of Transmission and Evolution of PEDV Variants and Co-Infections in Northeast China from 2011 to 2022.

Zhao F, Ma X, Yang J, Wei Z, Li J, Jiang Y Animals (Basel). 2024; 14(15).

PMID: 39123693 PMC: 11311072. DOI: 10.3390/ani14152168.

Research progress of porcine epidemic diarrhea virus S protein.

Luo H, Liang Z, Lin J, Wang Y, Liu Y, Mei K Front Microbiol. 2024; 15:1396894.

PMID: 38873162 PMC: 11169810. DOI: 10.3389/fmicb.2024.1396894.

References

Pospischil A, Hess R, Bachmann P . Light microscopy and ultrahistology of intestinal changes in pigs infected with epizootic diarrhoea virus (EVD): comparison with transmissible gastroenteritis (TGE) virus and porcine rotavirus infections. Zentralbl Veterinarmed B. 1981; 28(7):564-77. PMC: 7165649. DOI: 10.1111/j.1439-0450.1981.tb01774.x. View

Chasey D, CARTWRIGHT S . Virus-like particles associated with porcine epidemic diarrhoea. Res Vet Sci. 1978; 25(2):255-6. PMC: 7130664. View

Ducatelle R, Coussement W, Charlier G, Debouck P, Hoorens J . Three-dimensional sequential study of the intestinal surface in experimental porcine CV 777 coronavirus enteritis. Zentralbl Veterinarmed B. 1981; 28(6):483-93. PMC: 7165558. DOI: 10.1111/j.1439-0450.1981.tb01765.x. View

Takahashi K, Okada K, Ohshima K . An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan. Nihon Juigaku Zasshi. 1983; 45(6):829-32. DOI: 10.1292/jvms1939.45.829. View

Cox E, Hooyberghs J, Pensaert M . Sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus. Res Vet Sci. 1990; 48(2):165-9. PMC: 7130871. View

Wood E . An apparently new syndrome of porcine epidemic diarrhoea. Vet Rec. 1977; 100(12):243-4. DOI: 10.1136/vr.100.12.243. View

Kusanagi K, Kuwahara H, Katoh T, Nunoya T, Ishikawa Y, Samejima T . Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate. J Vet Med Sci. 1992; 54(2):313-8. DOI: 10.1292/jvms.54.313. View

Pensaert M, Debouck P, Reynolds D . An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV 777, and several coronaviruses. Arch Virol. 1981; 68(1):45-52. PMC: 7087258. DOI: 10.1007/BF01315166. View

Debouck P, Pensaert M . Experimental infection of pigs with a new porcine enteric coronavirus, CV 777. Am J Vet Res. 1980; 41(2):219-23. View

10.

Pensaert M, de Bouck P . A new coronavirus-like particle associated with diarrhea in swine. Arch Virol. 1978; 58(3):243-7. PMC: 7086830. DOI: 10.1007/BF01317606. View

11.

Turgeon D, Morin M, Jolette J, Higgins R, Marsolais G, Difranco E . Coronavirus-like particles associated with diarrhea in baby pigs in Quebec. Can Vet J. 1980; 21(3):100-xxiii. PMC: 1789692. View

12.

Hooper B, HAELTERMAN E . Growth of transmissible gastroenteritis virus in young pigs. Am J Vet Res. 1966; 27(116):286-91. View

13.

Horvath I, Mocsari E . Ultrastructural changes in the small intestinal epithelium of suckling pigs affected with a transmissible gastroenteritis (TGE)-like disease. Arch Virol. 1981; 68(2):103-13. PMC: 7086914. DOI: 10.1007/BF01314440. View