Molecular Site Assessment and Process Monitoring in Bioremediation and Natural Attenuation. off

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 1995 Jul 1

PMID 7486982

Citations 5

Authors

G S Sayler

A Layton

C Lajoie

J Bowman

M Tschantz

J T Fleming

Affiliations

Soon will be listed here.

Abstract

A variety of modern biotechnical approaches are available to assist in optimizing and controlling bioremediation processes. These approaches are broad-ranging, and may include genetic engineering to improve biodegradative performance, maintenance of the environment, and process monitoring and control. In addition to direct genetic engineering strategies, molecular diagnostic and monitoring technology using DNA gene probing methods and new quantitative mRNA analytical procedures allows direct analysis of degradative capacity, activity, and response under in situ conditions. Applications of these molecular approaches in process developments for trichloroethylene (TCE), polychlorinated biphenyls (PCB), and polynuclear aromatic hydrocarbons (PAH) bio-oxidation in soils, aquifer sediments, and ground-water treatment reactors have been demonstrated. Molecular genetic technologies permit not only the development of new processes for bioremediation, but also new process monitoring, control strategies, and molecular optimization paradigms that take full advantage of vast and diverse abilities of microorganisms to destroy problem chemicals.

Citing Articles

Monitoring gene expression in mixed microbial communities by using DNA microarrays.

Dennis P, Edwards E, Liss S, Fulthorpe R Appl Environ Microbiol. 2003; 69(2):769-78.

PMID: 12570994 PMC: 143597. DOI: 10.1128/AEM.69.2.769-778.2003.

Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique during in situ bioremediation.

Damborsky J, DAMBORSKA M, Stipek S, Jesenska A, Trantirek L, SKLENAR V Folia Microbiol (Praha). 2001; 45(1):35-40.

PMID: 11200669 DOI: 10.1007/BF02817447.

In situ, real-time catabolic gene expression: extraction and characterization of naphthalene dioxygenase mRNA transcripts from groundwater.

Wilson M, Bakermans C, Madsen E Appl Environ Microbiol. 1999; 65(1):80-7.

PMID: 9872763 PMC: 90986. DOI: 10.1128/AEM.65.1.80-87.1999.

Genotoxicity of bioremediated soils from the Reilly Tar site, St. Louis Park, Minnesota.

Hughes T, Claxton L, Brooks L, Warren S, Brenner R, Kremer F Environ Health Perspect. 1998; 106 Suppl 6:1427-33.

PMID: 9860901 PMC: 1533436. DOI: 10.1289/ehp.98106s61427.

Protein method for investigating mercuric reductase gene expression in aquatic environments.

Ogunseitan O Appl Environ Microbiol. 1998; 64(2):695-702.

PMID: 9464410 PMC: 106104. DOI: 10.1128/AEM.64.2.695-702.1998.

References

Ensley B . Biochemical diversity of trichloroethylene metabolism. Annu Rev Microbiol. 1991; 45:283-99. DOI: 10.1146/annurev.mi.45.100191.001435. View

King J, Digrazia P, Applegate B, Burlage R, Sanseverino J, Dunbar P . Rapid, sensitive bioluminescent reporter technology for naphthalene exposure and biodegradation. Science. 1990; 249(4970):778-81. DOI: 10.1126/science.249.4970.778. View

Lajoie C, Layton A, Sayler G . Cometabolic oxidation of polychlorinated biphenyls in soil with a surfactant-based field application vector. Appl Environ Microbiol. 1994; 60(8):2826-33. PMC: 201729. DOI: 10.1128/aem.60.8.2826-2833.1994. View

Layton A, Lajoie C, Easter J, Jernigan R, Beck M, Sayler G . Molecular diagnostics for polychlorinated biphenyl degradation in contaminated soils. Ann N Y Acad Sci. 1994; 721:407-22. DOI: 10.1111/j.1749-6632.1994.tb47412.x. View

Selifonova O, Burlage R, Barkay T . Bioluminescent sensors for detection of bioavailable Hg(II) in the environment. Appl Environ Microbiol. 1993; 59(9):3083-90. PMC: 182410. DOI: 10.1128/aem.59.9.3083-3090.1993. View

Shields M, Reagin M . Selection of a Pseudomonas cepacia strain constitutive for the degradation of trichloroethylene. Appl Environ Microbiol. 1992; 58(12):3977-83. PMC: 183214. DOI: 10.1128/aem.58.12.3977-3983.1992. View

Yates J, Mondello F . Sequence similarities in the genes encoding polychlorinated biphenyl degradation by Pseudomonas strain LB400 and Alcaligenes eutrophus H850. J Bacteriol. 1989; 171(3):1733-5. PMC: 209805. DOI: 10.1128/jb.171.3.1733-1735.1989. View

Sanseverino J, Applegate B, King J, Sayler G . Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene. Appl Environ Microbiol. 1993; 59(6):1931-7. PMC: 182185. DOI: 10.1128/aem.59.6.1931-1937.1993. View

Furukawa K, Hayase N, Taira K, Tomizuka N . Molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: some soil bacteria possess a highly conserved bph operon. J Bacteriol. 1989; 171(10):5467-72. PMC: 210385. DOI: 10.1128/jb.171.10.5467-5472.1989. View

10.

Heitzer A, Webb O, Thonnard J, Sayler G . Specific and quantitative assessment of naphthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium. Appl Environ Microbiol. 1992; 58(6):1839-46. PMC: 195692. DOI: 10.1128/aem.58.6.1839-1846.1992. View

11.

Phelps P, Agarwal S, Speitel G, Georgiou G . Methylosinus trichosporium OB3b Mutants Having Constitutive Expression of Soluble Methane Monooxygenase in the Presence of High Levels of Copper. Appl Environ Microbiol. 1992; 58(11):3701-8. PMC: 183163. DOI: 10.1128/aem.58.11.3701-3708.1992. View

12.

Asturias J, Timmis K . Three different 2,3-dihydroxybiphenyl-1,2-dioxygenase genes in the gram-positive polychlorobiphenyl-degrading bacterium Rhodococcus globerulus P6. J Bacteriol. 1993; 175(15):4631-40. PMC: 204914. DOI: 10.1128/jb.175.15.4631-4640.1993. View

13.

Lajoie C, Zylstra G, Deflaun M, Strom P . Development of field application vectors for bioremediation of soils contaminated with polychlorinated biphenyls. Appl Environ Microbiol. 1993; 59(6):1735-41. PMC: 182153. DOI: 10.1128/aem.59.6.1735-1741.1993. View

14.

Erickson B, Mondello F . Enhanced biodegradation of polychlorinated biphenyls after site-directed mutagenesis of a biphenyl dioxygenase gene. Appl Environ Microbiol. 1993; 59(11):3858-62. PMC: 182541. DOI: 10.1128/aem.59.11.3858-3862.1993. View

15.

Sanseverino J, Werner C, FLEMING J, Applegate B, King J, Sayler G . Molecular diagnostics of polycyclic aromatic hydrocarbon biodegradation in manufactured gas plant soils. Biodegradation. 1993; 4(4):303-21. DOI: 10.1007/BF00695976. View