Zinc-, Copper- and Cadmium-binding Protein in Ehrlich Ascites Tumour Cells

Overview

Journal Biochem J

Specialty Biochemistry

Date 1980 Jul 1

PMID 7458908

Citations 9

Authors

J Koch

S Wielgus

B Shankara

L A Saryan

C F Shaw

D H Petering

Affiliations

Soon will be listed here.

Abstract

The properties of Ehrlich ascites tumour cells exposed in vivo to cadmium were investigated as a function of the zinc status of the host animals. Tumour-cell growth was inhibited by cadmium in both zinc-sufficient and zinc-deficient animals. However, cells in zinc-sufficient tumours accumulate much less cadmium than those in deficient tumours. The subcellular distributions of cadmium and zinc do not depend on zinc status. Cadmium and zinc are bound to a low-molecular-weight protein with properties similar to metallothionein. Without exposure to cadmium, a zinc- and copper-binding protein is still present that behaves like a metallothionein. This protein can rapidly bind cadmium added to Ehrlich cells in vitro. It is shown that the zinc- and copper-binding protein contains free thiol groups. Ehrlich cells isolated from cadmium-treated animals are viable and show normal incorporation of uridine into RNA, but the cellular uptake of thymidine and its incorporation into DNA are inhibited.

Citing Articles

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Kraker A, Petering D Biol Trace Elem Res. 2013; 5(4-5):363-74.

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On the reactivity of metallothioneins with 5,5'-dithiobis-(2-nitrobenzoic acid).

Li T, Minkel D, Shaw 3rd C, Petering D Biochem J. 1981; 193(2):441-6.

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Ligand substitution reactions of metallothioneins with EDTA and apo-carbonic anhydrase.

Li T, Kraker A, Shaw 3rd C, Petering D Proc Natl Acad Sci U S A. 1980; 77(11):6334-8.

PMID: 6779278 PMC: 350278. DOI: 10.1073/pnas.77.11.6334.

Enzymatic activity of metal-binding proteins in epidermal cells.

Ito Y, Fukuyama K, Horie N, Epstein W Mol Cell Biochem. 1984; 60(2):183-8.

PMID: 6538644 DOI: 10.1007/BF00222488.

Roles of metallothionein and related proteins in metal metabolism and toxicity: problems and perspectives.

Petering D, Fowler B Environ Health Perspect. 1986; 65:217-24.

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