The Use of Sep-Pak C18 Cartridges During the Isolation of Gangliosides

Overview

Journal J Neurochem

Specialties Chemistry
Neurology

Date 1980 Jul 1

PMID 7452256

Citations 86

Authors

M A Williams

R H McCluer

Affiliations

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Abstract

The use of Sep-Pak C18 reverse-phase cartridges to adsorb gangliosides from aqueous solutions was studied. When upper phases formed from chloroform-methanol tissue extracts or aqueous salt solutions containing gangliosides are rapidly passed through the cartridges, the lipids are retained and the non-lipid components can be washed through. Gangliosides and other retained lipids can subsequently be eluted with methanol or chloroform-methanol

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