Article: Prevention of chromosomal breakage in Fanconi's anemia by cocultivation with normal cells

The frequency of aberrations in cultured lymphocytes from patients with Fanconi's anemia was significantly reduced when the cells were cocultivated with normal human lymphocytes. The results suggest that most of the chromosomal aberrations observed in cultured cells from Fanconi patients arise during cultivation and that the presence of normal cells prevents chromosomal damage by means of a hitherto unknown mechanism.

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References

1.

von Koskull H, AULA P . Nonrandom distribution of chromosome breaks in Fanconi's anemia. Cytogenet Cell Genet. 1973; 12(6):423-34. DOI: 10.1159/000130485. View

2.

Sasaki M . Is Fanconi's anaemia defective in a process essential to the repair of DNA cross links?. Nature. 1975; 257(5526):501-3. DOI: 10.1038/257501a0. View

3.

Joenje H, Eriksson A, Frants R, Arwert F, Houwen B . Erythrocyte superoxide-dismutase deficiency in Fanconi's anaemia. Lancet. 1978; 1(8057):204. DOI: 10.1016/s0140-6736(78)90632-3. View

4.

HUNGERFORD D . Leukocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KCl. Stain Technol. 1965; 40(6):333-8. DOI: 10.3109/10520296509116440. View

5.

Brown W, Epstein J, Little J . Progeria cells are stimulated to repair DNA by co-cultivation with normal cells. Exp Cell Res. 1976; 97(2):291-6. DOI: 10.1016/0014-4827(76)90619-4. View

6.

Germain D, Requin C . [Chromosome abnormalities in constitutional cytopenia]. Nouv Rev Fr Hematol. 1970; 10(1):107-17. View

7.

Hayashi K, Schmid W . The rate of sister chromatid exchanges parallel to spontaneous chromosome breakage in Fanconi's anemia and to trenimon-induced aberrations in human lymphocytes and fibroblasts. Humangenetik. 1975; 29(3):201-6. DOI: 10.1007/BF00297624. View

8.

Sperling K, Wegner R, Riehm H, Obe G . Frequency and distribution of sister-chromatid exchanges in a case of Fanconi's anemia. Humangenetik. 1975; 27(3):227-30. DOI: 10.1007/BF00278349. View

9.

Nordenson I . Effect of superoxide dismutase and catalase on spontaneously occurring chromosome breaks in patients with Fanconi's anemia. Hereditas. 1977; 86(2):147-50. DOI: 10.1111/j.1601-5223.1977.tb01223.x. View

10.

BLOOM G, Warner S, GERALD P, DIAMOND L . Chromosome abnormalities in constitutional aplastic anemia. N Engl J Med. 1966; 274(1):8-14. DOI: 10.1056/NEJM196601062740102. View

11.

SCHROEDER T, Drings P . [Course study of Fanconi's anemia in an adult]. Verh Dtsch Ges Inn Med. 1973; 79:477-81. View

12.

Dutrillaux B, Couturier J, Viegas-Pequignot E, Schaison G . Localization chromatid breaks in Fanconi's anemia, using three consecutive stains. Hum Genet. 1977; 37(1):65-71. DOI: 10.1007/BF00293773. View

Prevention of Chromosomal Breakage in Fanconi's Anemia by Cocultivation with Normal Cells