Genetic Analysis of Tumorigenesis: VI. Chromosome Rearrangements in Tumors Derived from Diploid Premalignant Chinese Hamster Cells in Nude Mice

Overview

Journal Somatic Cell Genet

Specialty Genetics

Date 1980 Sep 1

PMID 7434146

Citations 5

Authors

R M Kitchin

R Sager

Affiliations

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Abstract

The chromosome constitution of CHEF/16 clones recovered from methylcellulose and of uncloned, tumor-derived CHEF/16 populations are compared. Nine of 11 clones recovered from methycellulose were initiated by diploid cells. Moreover, chromosomally diploid cells were still present in most CHEF/16 clones even after growth in anchorage-independent conditions. In contrast, none of the CHEF/16 cells recovered from tumors were diploid. Nonrandom chromosome changes were observed, but no specific chromosome alterations were consistently found in tumor-derived CHEF cells. Although CHEF/16 cells are uniformly tumorigenic in nude mice, each of 10 uncloned tumor-derived populations from inocula of 10(2), 10(4), and 10(6) CHEF/16 cells consisted of only 1-3 stemlines. Our results show that diploid CHEF/16 cells are premalignant and undergo karyotypic changes leading to successful and usually clonal establishment of tumors in nude mice.

Citing Articles

DNA transfer of focus- and tumor-forming ability into nontumorigenic CHEF cells.

Smith B, Anisowicz A, Chodosh L, Sager R Proc Natl Acad Sci U S A. 1982; 79(6):1964-8.

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Azacytidine-induced tumorigenesis of CHEF/18 cells: correlated DNA methylation and chromosome changes.

Harrison J, Anisowicz A, Gadi I, Raffeld M, Sager R Proc Natl Acad Sci U S A. 1983; 80(21):6606-10.

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Sager R, Gadi I, Stephens L, Grabowy C Proc Natl Acad Sci U S A. 1985; 82(20):7015-9.

PMID: 3863138 PMC: 391300. DOI: 10.1073/pnas.82.20.7015.

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Stenman G, Delorme E, Lau C, Sager R Proc Natl Acad Sci U S A. 1987; 84(1):184-8.

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PMID: 3321067 PMC: 299699. DOI: 10.1073/pnas.84.24.9099.