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Coding and Spacer Sequences in the 5.8S-2S Region of Sciara Coprophila Ribosomal DNA

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1980 Aug 25
PMID 7433100
Citations 19
Authors
B R Jordan
R Jourdan
Affiliations
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Abstract

The sequence of 436 nucleotides around the region coding for 5.8S RNA in the Sciara coprophila rDNA transcription unit (1) has been determined. Regions coding for 5.8S and 2S RNAs have been identified; they are 80 - 90% homologous to the corresponding Drosophila sequences and are separated by a 22 nucleotide long spacer. This sequence as well as the two before the 5.8 and after the 2S coding region are very different from the corresponding Drosophila sequences. The main features reported in the Drosophila study (2) are however also found, i.e. all three spacers are very rich in A-T; the sequence of the internal spacer allows base pairing; 5.8S and 2S RNAs can pair through their 3' and 5' terminal regions respectively. The features previously proposed as processing sites in the Drosophila case are thus all found in Sciara in spite of very different spacer sequences.

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References
1.
Jordan B, Jourdan R, Jacq B . Late steps in the maturation of Drosophila 26 S ribosomal RNA: generation of 5-8 S and 2 S RNAs by cleavages occurring in the cytoplasm. J Mol Biol. 1976; 101(1):85-105. DOI: 10.1016/0022-2836(76)90067-x. View
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Pavlakis G, Jordan B, Wurst R, Vournakis J . Sequence and secondary structure of Drosophila melanogaster 5.8S and 2S rRNAs and of the processing site between them. Nucleic Acids Res. 1979; 7(8):2213-38. PMC: 342381. DOI: 10.1093/nar/7.8.2213. View