Automated Colorimetric Estimation of Glycosylated Haemoglobins

Overview

Journal Clin Chim Acta

Specialty Biochemistry

Date 1980 Sep 8

PMID 7408208

Citations 5

Authors

J M Burrin

R Worth

L A ASHWORTH

S Currie

K G Alberti

Affiliations

Soon will be listed here.

Abstract

The measurement of glycosylated haemoglobin is now widely used as a guide to glycaemic control in patients with diabetes mellitus. Present methods for measurement of glycosylated haemoglobin are either laborious, expensive or both. We describe a precise and inexpensive method for the automated analysis of glycosylated haemoglobin based on a colorimetric technique. The within-batch coefficient of variation ranged from 2.0 to 4.8% and between-batch was less than 11.0%. At least 200 samples can be analysed per day.

Citing Articles

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Ziegelhoffer-Mihalovicova B, Waczulikova I, Sikurova L, Styk J, Carsky J, Ziegelhoffer A Mol Cell Biochem. 2003; 249(1-2):175-82.

PMID: 12956413 DOI: 10.1023/a:1024703226034.

Mechanisms that may be involved in calcium tolerance of the diabetic heart.

Ziegelhoffer A, Ravingerova T, Styk J, Sebokova J, Waczulikova I, Breier A Mol Cell Biochem. 1997; 176(1-2):191-8.

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A collaborative study of the measurement of glycosylated haemoglobin by several methods in seven laboratories in the United Kingdom.

Boucher B, Burrin J, GOULD B, John P, Lewis G, Owens C Diabetologia. 1983; 24(4):265-71.

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[Comparison of various methods for determining glycosylated hemoglobins].

Schlebusch H, Sorger M, Burisch H Klin Wochenschr. 1984; 62(16):787-92.

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Intermediate acting insulin given at bedtime: effect on blood glucose concentrations before and after breakfast.

Francis A, Home P, Hanning I, Alberti K, Tunbridge W Br Med J (Clin Res Ed). 1983; 286(6372):1173-6.

PMID: 6404377 PMC: 1547399. DOI: 10.1136/bmj.286.6372.1173.