Detection of Teratogenic Compounds Using Differentiating Embryonic Cells in Culture

Overview

Journal In Vitro

Specialties Anatomy & Histology
Cell Biology

Date 1980 Apr 1

PMID 7399542

Citations 12

Authors

A L WILK

J H Greenberg

E A Horigan

R M Pratt

G R Martin

Affiliations

Soon will be listed here.

Abstract

Methods are described for screening for teratogenic compounds using differentiating neural crest and prechondrogenic limb bud mesenchyme cells in culture. Substances to be tested are either added directly to the culture medium or are combined in a dialysis bag with the postmitochondrial fraction from rat liver and certain cofactors. In the latter case, the compound and its metabolites are gradually released into the medium from the dialysis bag. The results obtained with 14 compounds demonstrate a positive relationship between teratogenicity in vivo and alterations in the growth or the differentiation of the cultured cells.

Citing Articles

Characterization of cytoskeletal and neuronal markers in micromass cultures of rat embryonic midbrain cells.

Whittaker S, Wroble J, Silbernagel S, Faustman E Cell Biol Toxicol. 1993; 9(4):359-75.

PMID: 8039012 DOI: 10.1007/BF00754465.

Transplacental movement of inorganic lead in early and late gestation in the mouse.

Danielsson B, Dencker L, Lindgren A Arch Toxicol. 1983; 54(2):97-107.

PMID: 6651530 DOI: 10.1007/BF01261379.

Microtiter micromass cultures of limb-bud mesenchymal cells.

Paulsen D, Solursh M In Vitro Cell Dev Biol. 1988; 24(2):138-47.

PMID: 3343192 DOI: 10.1007/BF02623891.

Teratological research using in vitro systems. II. Rodent limb bud culture system.

Friedman L Environ Health Perspect. 1987; 72:211-9.

PMID: 3304997 PMC: 1474665. DOI: 10.1289/ehp.8772211.

Effects of anticonvulsants on cholinergic and GABAergic properties in the neuronal cell clone NG108-15.

Searles C, Slesinger P, Singer H Neurochem Res. 1988; 13(10):1007-13.

PMID: 3216946 DOI: 10.1007/BF00970776.

References

Hamburger V, HAMILTON H . A series of normal stages in the development of the chick embryo. J Morphol. 2014; 88(1):49-92. View

UMANSKY R . The effect of cell population density on the developmental fate of reaggregating mouse limb bud mesenchyme. Dev Biol. 1966; 13(1):31-56. DOI: 10.1016/0012-1606(66)90048-0. View

Bridges B . Short term screening tests for carcinogens. Nature. 1976; 261(5557):195-200. DOI: 10.1038/261195a0. View

Gibson J, BECKER B . Teratogenic effects of diphenylhydantoin in Swiss-Webster and A-J mice. Proc Soc Exp Biol Med. 1968; 128(3):905-9. DOI: 10.3181/00379727-128-33154. View

McClure H, WILK A, Horigan E, Pratt R . Induction of craniofacial malformations in rhesus monkeys (Macaca mulatta) with cyclophosphamide. Cleft Palate J. 1979; 16(3):248-56. View

WILK A . Production of fetal rat malformations by norchlorcyclizine and chlorcyclizine after intrauterine application. Teratology. 1969; 2(1):55-65. DOI: 10.1002/tera.1420020108. View

Kimmel C, Wilson J, Schumacher H . Studies on metabolism and identification of the causative agent in aspirin teratogenesis in rats. Teratology. 1971; 4(1):15-24. DOI: 10.1002/tera.1420040104. View

Piez K . Cross-linking of collagen and elastin. Annu Rev Biochem. 1968; 37:547-70. DOI: 10.1146/annurev.bi.37.070168.002555. View

Steffek A, King C, WILK A . Abortive effects and comparative metabolism of chlorcyclizine in various mammalian species. Teratology. 1968; 1(4):399-406. DOI: 10.1002/tera.1420010407. View

10.

WILK A, King C, Horigan E, Steffek A . Metabolism of -aminopropionitrile and its teratogenic activity in rats. Teratology. 1972; 5(1):41-8. DOI: 10.1002/tera.1420050108. View

11.

WILK A, King C, Pratt R . Chlorcyclizine induction of cleft palate in the rat: degradation of palatal glycosaminoglycans. Teratology. 1978; 18(2):199-209. DOI: 10.1002/tera.1420180205. View

12.

Allen L, Creaven P . In vitro activation of isophosphamide (NSC-109724), a new oxazaphosphorine, by rat liver microsomes. Cancer Chemother Rep. 1972; 56(5):603-10. View

13.

GOETINCK P, Pennypacker J, Royal P . Proteochondroitin sulfate synthesis and chondrogenic expression. Exp Cell Res. 1974; 87(2):241-8. DOI: 10.1016/0014-4827(74)90476-5. View

14.

Kochhar D . The use of in vitro procedures in teratology. Teratology. 1975; 11(3):273-87. DOI: 10.1002/tera.1420110307. View

15.

Madle S, Obe G . In vitro testing of an indirect mutagen (cyclophosphamide) with human leukocyte cultures: activation with liver microsomes and use of a dialysis bag. Mutat Res. 1977; 56(1):101-4. DOI: 10.1016/0027-5107(77)90250-0. View

16.

Rutter W, Pictet R, MORRIS P . Toward molecular mechanisms of developmental processes. Annu Rev Biochem. 1973; 42:601-46. DOI: 10.1146/annurev.bi.42.070173.003125. View

17.

Hassell J, Pennypacker J, Lewis C . Chondrogenesis and cell proliferation in limb bud cell cultures treated with cytosine arabinoside and vitamin A. Exp Cell Res. 1978; 112(2):409-17. DOI: 10.1016/0014-4827(78)90223-9. View

18.

Ames B, Durston W, Yamasaki E, Lee F . Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci U S A. 1973; 70(8):2281-5. PMC: 433718. DOI: 10.1073/pnas.70.8.2281. View

19.

Greenberg J, Schrier B . Development of choline acetyltransferase activity in chick cranial neural crest cells in culture. Dev Biol. 1977; 61(1):86-93. DOI: 10.1016/0012-1606(77)90344-x. View

20.

Steffek A, Verrusio A, WATKINS C . Cleft palate in rodents after maternal treatment with various lathyrogenic agents. Teratology. 1972; 5(1):33-8. DOI: 10.1002/tera.1420050107. View