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Cell-free Synthesis of Glial Fibrillary Acidic Protein

Overview
Journal Neurochem Res
Specialties Chemistry
Neurology
Date 1980 May 1
PMID 7393381
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Abstract

Glial fibrillary acidic (GFA) protein has been synthesized in an RNA-dependent cell-free system derived from rabbit reticulocytes. The cell-free synthesized product appears to have the same size as GFA protein isolated from bovine spinal cord, thus showing that GFA protein does not undergo detectable proteolytic processing.

References
1.
Cleveland D, Fischer S, Kirschner M, Laemmli U . Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977; 252(3):1102-6. View

2.
Dahl D, Bignami A . Glial fibrillary acidic protein from normal and gliosed human brain. Demonstration of multiple related polypeptides. Biochim Biophys Acta. 1975; 386(1):41-51. DOI: 10.1016/0005-2795(75)90244-5. View

3.
Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

4.
Pelham H, Jackson R . An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976; 67(1):247-56. DOI: 10.1111/j.1432-1033.1976.tb10656.x. View

5.
Eng L, Rubinstein L . Contribution of immunohistochemistry to diagnostic problems of human cerebral tumors. J Histochem Cytochem. 1978; 26(7):513-22. DOI: 10.1177/26.7.357640. View