A Radioimmunoassay for Quantitation of Secretory Component

Overview

Journal Gastroenterol Jpn

Specialty Gastroenterology

Date 1980 Apr 1

PMID 7380167

Citations 3

Authors

K Kunishi

Affiliations

Soon will be listed here.

Abstract

Secretory component (SC) isolated from human colostrum was radioiodinated with chloramin-T method and it was subjected to a radioimmunoassay for quantitation of SC. A monospecific rabbit anti-sera against human SC which could react with free SC as well as immunoglobulin bound SC was used for the assay. The present method can measure as low as 300 ng/ml of SC. The level (mean +/- 2 S.D.) of serum SC in healthy subjects was determined to be 31.3 +/- 7.2 micrograms/ml. Studies with Sephadex G-200 gel filtration demonstrated that SC in the normal sera existed mostly as a form of SC-IgA. In contrast, free SC was never detected in them.

Citing Articles

Studies on secretory component in digestive disease. IV. Levels of serum secretory component in liver disease.

Kunishi K, Arima T, Yasuhara T, Narumoto J, Suwaki K, Nagashima H Gastroenterol Jpn. 1980; 15(5):448-51.

PMID: 7439619 DOI: 10.1007/BF02773907.

Studies on secretory component in digestive disease. III. Levels of serum secretory component in digestive disease.

Kunishi K Gastroenterol Jpn. 1980; 15(5):444-7.

PMID: 7439618 DOI: 10.1007/BF02773906.

Serum secretory component level in myeloma patients with disorders of the secretory surface.

Kunishi K, Arima T, Yasuhara T, Narumoto J, Suwaki K, Kita S Gastroenterol Jpn. 1980; 15(2):148-51.

PMID: 6769740 DOI: 10.1007/BF02774929.

References

Tourville D, Adler R, Bienenstock J, TOMASI Jr T . The human secretory immunoglobulin system: immunohistoligical localization of gamma A, secretory "piece," and lactoferrin in normal human tissues. J Exp Med. 1969; 129(2):411-29. PMC: 2138606. DOI: 10.1084/jem.129.2.411. View

Brown W, Isobe Y, Nakane P . Studies on translocation of immunoglobulins across intestinal epithelium. II. Immunoelectron-microscopic localization of immunoglobulins and secretory component in human intestinal mucosa. Gastroenterology. 1976; 71(6):985-95. View

Asofski R, BINAGHI R, Edelman G, Goodman H, Heremans J, Hood L . An extension of the nomenclature for immunoglobulins. Immunochemistry. 1970; 7(5):497-500. DOI: 10.1016/0019-2791(70)90232-6. View

Thompson R, Asquith P . Quantitation of exocrine IgA in human serum in health and disease. Clin Exp Immunol. 1970; 7(4):491-500. PMC: 1712853. View

Halpern M, Koshland M . Noval subunit in secretory IgA. Nature. 1970; 228(5278):1276-8. DOI: 10.1038/2281276a0. View

Brandtzaeg P . Mucosal and glandular distribution of immunoglobulin components: differential localization of free and bound SC in secretory epithelial cells. J Immunol. 1974; 112(4):1553-9. View

Jackson G, Vaerman J . High levels of secretory IgA and free secretory component in the serum of rats with bile duct obstruction. J Exp Med. 1978; 147(3):934-9. PMC: 2184194. DOI: 10.1084/jem.147.3.934. View

SCHALCH D, Parker M . A SENSITIVE DOUBLE ANTIBODY IMMUNOASSAY FOR HUMAN GROWTH HORMONE IN PLASMA. Nature. 1964; 203:1141-2. DOI: 10.1038/2031141a0. View

Samaan N, YEN S, Friesen H, Pearson O . Serum placental lactogen levels during pregnancy and in trophoblastic disease. J Clin Endocrinol Metab. 1966; 26(12):1303-8. DOI: 10.1210/jcem-26-12-1303. View

10.

Radl J, Klein F, van den Berg P, de Bruyn A, HIJMANS W . Binding of secretory piece to polymeric IgA and IgM paraproteins in vitro. Immunology. 1971; 20(5):843-52. PMC: 1455876. View

11.

Thompson R, Asquith P, COOKE W . Secretory IgA in the serum. Lancet. 1969; 2(7619):517-9. DOI: 10.1016/s0140-6736(69)90217-7. View

12.

Arima T, Motoyama Y, Nagata K, Kondo T . Serum glycoproteins in the liver diseases. II. Radioimmunoassay of alpha-1 acid glycoprotein. Gastroenterol Jpn. 1976; 11(4):307-12. View

13.

GREENWOOD F, Hunter W, GLOVER J . THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963; 89:114-23. PMC: 1202279. DOI: 10.1042/bj0890114. View

14.

Thompson R . Secretory piece linked to IgM in individuals deficient in IgA. Nature. 1970; 226(5249):946-8. DOI: 10.1038/226946a0. View

15.

Waldman R, Mach J, Stella M, Rowe D . Secretory IgA in human serum. J Immunol. 1970; 105(1):43-7. View

16.

Kobayashi K . Studies on human secretory IgA comparative studies of the IgA-bound secretory piece and the free secretory piece protein. Immunochemistry. 1971; 8(9):785-800. DOI: 10.1016/0019-2791(71)90446-0. View

17.

Brandtzaeg P . Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique. Immunology. 1974; 26(6):1101-14. PMC: 1423367. View

18.

Weicker J, Underdown B . A study of the association of human secretory component with IgA and IgM proteins. J Immunol. 1975; 114(4):1337-44. View

19.

TOMASI Jr T, ZIGELBAUM S . THE SELECTIVE OCCURENCE OF GAMMA-1A GLOBULINS IN CERTAIN BODY FLUIDS. J Clin Invest. 1963; 42:1552-60. PMC: 289434. DOI: 10.1172/JCI104840. View