Structure of the Regular Surface Layer of Sporosarcina Ureae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Apr 1

PMID 7372574

Citations 15

Authors

M Stewart

T J Beveridge

Affiliations

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Abstract

Optical diffraction and computer image processing of electron micrographs were employed to analyze the structure of the regular surface layer of Sporosarcina ureae at high resolution. Negatively stained preparations of regular surface layer fragments showed two types of tetragonal pattern, each having p4 symmetry in projection with a = 12.8 nm. Although the two patterns differed greatly in overall appearance, both had a common pattern of areas of high stain density which we interpret as arising from gaps or holes in the structure. We speculate that these holes may be related to a protective role of the regular surface layer, whereby hostile environmental agents (such as muramidases) larger than about 2 nm would be screened from the underlying layers of the bacterial surface, while the free passage of nutrients and waste products into and out of the cell would still be allowed.

Citing Articles

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Purification and disposition of a surface protein associated with virulence of Aeromonas salmonicida.

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Ultrastructure of square bacteria from a brine pool in Southern Sinai.

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Structure of the regular surface layer of Aquaspirillum serpens MW5.

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PMID: 7061396 PMC: 220119. DOI: 10.1128/jb.150.1.348-357.1982.

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