Hybridoma Autoantibodies to DNA

Overview

Journal J Immunol

Specialty Allergy & Immunology

Date 1980 Mar 1

PMID 7358989

Citations 23

Authors

C Andrzejewski Jr

B D Stollar

T M Lalor

R S Schwartz

Affiliations

Soon will be listed here.

Abstract

MRL/1 mice develop a severe form of systemic lupus erythematosus and spontaneously produce high levels of autoantibodies to DNA. Spleen cells from these animals were used as fusion partners to prepare hybridomas that produce autoantibodies to DNA. Since exogenous immunization was not employed, the hybridoma antibodies were derived from populations of B cells that spontaneously produced autoantibodies. Each hybridoma autoantibody had a distinctive binding specificity with S-DNA, N-DNA, or oligonucleotides.

Citing Articles

Mechanisms of autoantibody production in autoimmune MRL mice.

Pisetsky D, McCarty G, Peters D J Exp Med. 1980; 152(5):1302-10.

PMID: 7430950 PMC: 2185992. DOI: 10.1084/jem.152.5.1302.

Production of autoantibodies by human-human hybridomas.

Shoenfeld Y, Gabriels J, Silberstein L, Furie B, Furie B, Stollar B J Clin Invest. 1982; 70(1):205-8.

PMID: 7085884 PMC: 370244. DOI: 10.1172/jci110595.

Transferring DNA from electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose: properties of nucleosomes along mouse satellite DNA.

Reudelhuber T, Ball D, Davis A, Garrard W Nucleic Acids Res. 1982; 10(4):1311-25.

PMID: 7071014 PMC: 320527. DOI: 10.1093/nar/10.4.1311.

Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids.

Lafer E, Rauch J, Andrzejewski Jr C, Mudd D, Furie B, Schwartz R J Exp Med. 1981; 153(4):897-909.

PMID: 6972993 PMC: 2186121. DOI: 10.1084/jem.153.4.897.

Idiotypic cross-reactions of monoclonal human lupus autoantibodies.

Shoenfeld Y, Isenberg D, Rauch J, Madaio M, Stollar B, Schwartz R J Exp Med. 1983; 158(3):718-30.

PMID: 6886627 PMC: 2187101. DOI: 10.1084/jem.158.3.718.