Free Calcium in Xenopus Embryos Measured with Ion-selective Microelectrodes

Overview

Journal Nature

Specialty Science

Date 1980 Feb 14

PMID 7354852

Citations 44

Authors

T J Rink

R Y Tsien

A E Warner

Affiliations

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Abstract

A regulatory role for intracellular free calcium has been suggested in cell division and intercellular communication via gap junctions. Previous measurements of cytoplasmic free Ca2+ during cell division and uncoupling have been made with the Ca2+-sensitive photoprotein aequorin. We now report experiments using Ca2+-sensitive microelectrodes to monitor free Ca2+ in the cells and intercellular fluid of early embryos of the amphibian Xenopus laevis. In addition to measuring basal levels, we have looked for changes in free Ca2+ during cell division and during electrical uncoupling of the normally coupled embryonic cells, induced by acidification of the intracellular medium.

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