Alpha-methylhistamine Methylation by Histamine Methyltransferase

Overview

Journal Agents Actions

Specialty Pharmacology

Date 1981 Nov 1

PMID 7337069

Citations 2

Authors

L B Hough

J K Khandelwal

T W Mittag

Affiliations

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Abstract

Enzymatic methylation of histamine and analogues by histamine methyltransferase (HMT) was studied. Incubation of histamine or alpha-methylhistamine with HMT and S-adenosylmethionine was shown by GC-MS to yield tele-methyl and alpha-methyl-tele-methyl-histamine, respectively. Kinetic analysis of the reactions indicated that both substrates exhibited equivalent Vmax values, but that histamine's Km was ten-fold lower than that of alpha-methylhistamine. These results indicate that alpha-methylhistamine can be methylated as effectively as histamine, and may be a useful tool in further studies of HMT.

Citing Articles

Gastric histamine methyltransferase: different methylation rates for enantiomers of side-chain methylated histamine analogues using a highly purified enzyme preparation.

Barth H, Schunack W, Crombach M, Lorenz W Agents Actions. 1984; 14(3-4):346-50.

PMID: 6731176 DOI: 10.1007/BF01973824.

Reliability and practicability of the fluorometric-fluoroenzymatic histamine determination in pathogenetic studies on peptic ulcer: detection limits and problems with specificity.

Lorenz W, Thon K, Neugebauer E, Stoltzing H, Ohmann C, Weber D Agents Actions. 1987; 21(1-2):1-25.

PMID: 3630852 DOI: 10.1007/BF01974915.

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