The Determination of Labetalol in Plasma by High-performance Liquid Chromatography Using Fluorescence Detection

Overview

Journal J Pharmacol Methods

Specialties Pharmacology
Toxicology

Date 1981 Dec 1

PMID 7334812

Citations 4

Authors

P A MEREDITH

D McSharry

H L Elliott

J L Reid

Affiliations

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Abstract

A sensitive and specific assay for labetalol is described. The method employed is reverse phase high-performance liquid chromatography (HPLC) with fluorescence detection. Using 1 ml of plasma, the limit of detection of the assay was 8 ng/ml and the coefficient of variation over a typical calibration range is 5.5%. Various drugs that might be coadministered with labetalol are shown not to interfere in the assay. The method has been employed to study the pharmacokinetics of labetalol following intravenous and oral administration.

Citing Articles

Labetalol disposition and concentration-effect relationships during pregnancy.

Rubin P, Butters L, Kelman A, Fitzsimons C, Reid J Br J Clin Pharmacol. 1983; 15(4):465-70.

PMID: 6849783 PMC: 1427790. DOI: 10.1111/j.1365-2125.1983.tb01531.x.

"Third drug" trial: comparative study of antihypertensive agents added to treatment when blood pressure remains uncontrolled by a beta blocker plus thiazide diuretic.

McAreavey D, Ramsey L, Latham L, McLAREN A, LORIMER A, Reid J Br Med J (Clin Res Ed). 1984; 288(6411):106-11.

PMID: 6419809 PMC: 1443950. DOI: 10.1136/bmj.288.6411.106.

Comparison of the clinical pharmacokinetics and concentration-effect relationships for medroxalol and labetalol.

Elliott H, MEREDITH P, Sumner D, Reid J Br J Clin Pharmacol. 1984; 17(5):573-8.

PMID: 6145441 PMC: 1463447. DOI: 10.1111/j.1365-2125.1984.tb02392.x.

Comparison of medroxalol and labetalol, drugs with combined alpha- and beta-adrenoceptor antagonist properties.

Elliott H, McLean K, MEREDITH P, Sumner D, Reid J Br J Clin Pharmacol. 1984; 17(5):565-72.

PMID: 6145440 PMC: 1463449. DOI: 10.1111/j.1365-2125.1984.tb02391.x.