A Two-step Procedure for the Purificaton of Hepatitis B Surface Antigen (HBsAg)

Overview

Journal Vox Sang

Specialty Hematology

Date 1981 Jan 1

PMID 7331285

Citations 4

Authors

M Einarsson

L Kaplan

H Pertoft

Affiliations

Soon will be listed here.

Abstract

Hepatitis B surface antigen (HBsAg) was purified by a simple, rapid, two-step procedure comprising chromatography on dextran sulfate-Sepharose 4B, followed by preparative rate zonal centrifugation on a sucrose gradient. A 230-fold purification of HBsAg was achieved with a yield of about 80%.

Citing Articles

Rapid and Robust Continuous Purification of High-Titer Hepatitis B Virus for In Vitro and In Vivo Applications.

Wettengel J, Linden B, Esser K, Laue M, Burwitz B, Protzer U Viruses. 2021; 13(8).

PMID: 34452368 PMC: 8402639. DOI: 10.3390/v13081503.

Detection of cellular and humoral immunity to hepatitis B surface antigen (HBsAg) in asymptomatic HBsAg carriers.

Sylvan S, Hellstrom U, Lundbergh P Clin Exp Immunol. 1985; 62(2):288-95.

PMID: 3878745 PMC: 1577451.

Characterization of cell mediated immune responses to the hepatitis B core protein in man.

Sylvan S, Hellstrom U, Flehmig B Clin Exp Immunol. 1987; 68(2):233-41.

PMID: 3652517 PMC: 1542711.

HBsAg-induced interferon-gamma secretion in T cells from asymptomatic HBsAg carriers and HB-immune donors in vitro.

Sylvan S, Hellstrom U Immunology. 1990; 70(2):197-202.

PMID: 2115499 PMC: 1384193.