The Effects of Barium on the Electrical Properties of the Basolateral Membrane in Proximal Tubule

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1981 Dec 1

PMID 7329454

Citations 21

Authors

G Planelles

J Teulon

T Anagnostopoulos

Affiliations

Soon will be listed here.

Abstract

We studied the effects of millimolar Ba2+ concentrations on the properties of the basolateral membrane of the proximal tubule in Necturus kidney. Ba2+ was added in the peritubular perfusate by means of capillary microperfusion experiments. Basolateral membrane p.d. and conductance were continuously monitored in single tubules (their lumen was filled with oil) during reversible application of Ba2+ in peritubular fluid. The effect was membrane depolarization by 14mV and a decrease of membrane conductance to 63% of control values. This association strongly suggests that the main effect of Ba2+ is a decrease of the partial conductance to K+ at the basolateral membrane. However, in other experiments, performed also in oil-filled tubules, Ba2+ was shown to decrease intracellular K+ activity, recorded continuously by means of double-barreled (selective vs non selective) K+ microelectrodes. This and other, indirect evidence support the hypothesis that Ba2+ elicits also a small increase of the partial conductance to sodium at the basolateral membrane.

Citing Articles

Molecular aspects of structure, gating, and physiology of pH-sensitive background K2P and Kir K+-transport channels.

Sepulveda F, Cid L, Teulon J, Niemeyer M Physiol Rev. 2014; 95(1):179-217.

PMID: 25540142 PMC: 4281587. DOI: 10.1152/physrev.00016.2014.

Chloride transport in the renal proximal tubule.

Planelles G Pflugers Arch. 2004; 448(6):561-70.

PMID: 15258765 DOI: 10.1007/s00424-004-1309-y.

Extracellular ATP raises cytosolic calcium and activates basolateral chloride conductance in Necturus proximal tubule.

Bouyer P, Paulais M, Cougnon M, Hulin P, Anagnostopoulos T, Planelles G J Physiol. 1998; 510 ( Pt 2):535-48.

PMID: 9706002 PMC: 2231047. DOI: 10.1111/j.1469-7793.1998.535bk.x.

Change of apparent stoichiometry of proximal-tubule Na(+)-HCO3- cotransport upon experimental reversal of its orientation.

Planelles G, Thomas S, Anagnostopoulos T Proc Natl Acad Sci U S A. 1993; 90(15):7406-10.

PMID: 8346263 PMC: 47146. DOI: 10.1073/pnas.90.15.7406.

Triflocin, a novel inhibitor for the Na-HCO3 symport in the proximal tubule.

Belachgar F, Hulin P, Anagnostopoulos T, Planelles G Br J Pharmacol. 1994; 112(2):465-70.

PMID: 8075865 PMC: 1910392. DOI: 10.1111/j.1476-5381.1994.tb13096.x.

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