Method for the Removal of Selected Cells from Cytological Smear Preparations for Transmission Electron Microscopy

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1981 Dec 1

PMID 7328181

Citations 2

Authors

J Mather

C.M. Stanbridge

E B Butler

Affiliations

Soon will be listed here.

Abstract

The method described depends on fixing additional cytology smear preparations with glutaraldehyde to be used, if needed. Removal of cells from the slide makes use of partially polymerised resins in the usual way but a paper marker is used to localise the area in the resin block which contains the cells to be examined. Slides are placed in a freezing cabinet to facilitate easy separation of the resin block.

Citing Articles

An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis.

Kumar S, Filippi M J Vis Exp. 2016; (116).

PMID: 27768053 PMC: 5092190. DOI: 10.3791/54724.

An efficient and reproducible process for transmission electron microscopy (TEM) of rare cell populations.

Kumar S, Ciraolo G, Hinge A, Filippi M J Immunol Methods. 2013; 404:87-90.

PMID: 24291346 PMC: 3943659. DOI: 10.1016/j.jim.2013.11.025.

References

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