A Theoretical Study on the Sucrose Gap Technique As Applied to Multicellular Muscle Preparations. I. Saline-sucrose Interdiffusion

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1981 Dec 1

PMID 7326323

Citations 11

Authors

E Lammel

Affiliations

Soon will be listed here.

Abstract

Voltage-clamp analysis of membrane currents in multicellular muscle preparations by means of the sucrose gap method is complicated by diffusion of saline and sucrose in the interstitial fluid spaces. This paper is the first part of a theoretical study made to analyze electrical events related to this diffusion process. Concentration profiles of ions and sucrose (both axial and radial) were computed by solving diffusion equations with boundary conditions appropriate for the different types of preparations and experimental arrangements used. In addition to steady-state solutions, analytical expressions were derived that describe the time-course with which concentration profiles become established after a stepwise change of the solute concentration in one of the compartments of the sucrose gap apparatus. The model accounts for the presence of an endothelial surface layer, or endocardium, which acts as an external diffusion barrier and is important in determining concentration gradients of solutes within heart cell preparations. Results of numerical computations dealing with several cases of experimental interest are presented.

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References

Rougier O, Vassort G, Stampfli R . Voltage clamp experiments on frog atrial heart muscle fibres with the sucrose gap technique. Pflugers Arch Gesamte Physiol Menschen Tiere. 1968; 301(2):91-108. DOI: 10.1007/BF00362729. View

Berger W . [THE DOUBLE SACCHAROSE COMPARTMENT TECHNIC: A METHOD FOR STUDYING THE MEMBRANE POTENTIAL AND MEMBRANE PROPERTIES OF SMOOTH MUSCLE]. Pflugers Arch Gesamte Physiol Menschen Tiere. 1963; 277:570-6. View

Brown H, Noble S . Membrane currents underlying delayed rectification and pace-maker activity in frog atrial muscle. J Physiol. 1969; 204(3):717-36. PMC: 1351582. DOI: 10.1113/jphysiol.1969.sp008940. View

Adrian R, Chandler W, HODGKIN A . The kinetics of mechanical activation in frog muscle. J Physiol. 1969; 204(1):207-30. PMC: 1351604. DOI: 10.1113/jphysiol.1969.sp008909. View

Schneider M . Linear electrical properties of the transverse tubules and surface membrane of skeletal muscle fibers. J Gen Physiol. 1970; 56(5):640-71. PMC: 2225972. DOI: 10.1085/jgp.56.5.640. View

Chapman R, NIEDERGERKE R . Effects of calcium on the contraction of the hypodynamic frog heart. J Physiol. 1970; 211(2):389-421. PMC: 1395690. DOI: 10.1113/jphysiol.1970.sp009284. View

Beeler Jr G, Reuter H . Voltage clamp experiments on ventricular myocarial fibres. J Physiol. 1970; 207(1):165-90. PMC: 1348699. DOI: 10.1113/jphysiol.1970.sp009055. View

TARR M, Trank J . Equivalent circuit of frog atrial tissue as determined by voltage clamp-unclamp experiments. J Gen Physiol. 1971; 58(5):511-22. PMC: 2226042. DOI: 10.1085/jgp.58.5.511. View

Morad M, ORKAND R . Excitation-concentration coupling in frog ventricle: evidence from voltage clamp studies. J Physiol. 1971; 219(1):167-89. PMC: 1331624. DOI: 10.1113/jphysiol.1971.sp009656. View

10.

NEW W, TRAUTWEIN W . Inward membrane currents in mammalian myocardium. Pflugers Arch. 1972; 334(1):1-23. DOI: 10.1007/BF00585997. View

11.

PAGE S, NIEDERGERKE R . Structures of physiological interest in the frog heart ventricle. J Cell Sci. 1972; 11(1):179-203. DOI: 10.1242/jcs.11.1.179. View

12.

Kootsey J, Johnson E . Voltage clamp of cardiac muscle. A theoretical analysis of early currents in the single sucrose gap. Biophys J. 1972; 12(11):1496-508. PMC: 1484190. DOI: 10.1016/S0006-3495(72)86177-0. View

13.

Harrington L, Johnson E . Voltage clamp of cardiac muscle in a double sucrose gap. A feasibility study. Biophys J. 1973; 13(7):626-47. PMC: 1484318. DOI: 10.1016/S0006-3495(73)86013-8. View

14.

TARR M, TRANK J . An assessment of the double sucrose-gap voltage clamp technique as applied to frog atrial muscle. Biophys J. 1974; 14(9):627-43. PMC: 1334560. DOI: 10.1016/S0006-3495(74)85940-0. View

15.

McGuigan J . Some limitations of the double sucrose gap, and its use in a study of the slow outward current in mammalian ventricular muscle. J Physiol. 1974; 240(3):775-806. PMC: 1331006. DOI: 10.1113/jphysiol.1974.sp010634. View

16.

Lammel E, NIEDERGERKE R, Page S . Analysis of a rapid twitch facilitation in the frog heart. Proc R Soc Lond B Biol Sci. 1975; 189(1097):577-90. DOI: 10.1098/rspb.1975.0073. View

17.

Ramon F, Anderson N, Joyner R, Moore J . Axon voltage-clamp simulations. A multicellular preparation. Biophys J. 1975; 15(1):55-69. PMC: 1334610. DOI: 10.1016/S0006-3495(75)85791-2. View

18.

Johnson E, Lieberman M . Heart: excitation and contraction. Annu Rev Physiol. 1971; 33:479-532. DOI: 10.1146/annurev.ph.33.030171.002403. View

19.

Poindessault J, Duval A, Leoty C . Voltage clamp with double sucrose gap technique. External series resistance compensation. Biophys J. 1976; 16(2 Pt 1):105-20. PMC: 1334822. DOI: 10.1016/s0006-3495(76)85668-8. View

20.

DE HEMPTINNE A . Voltage clamp analysis in isolated cardiac fibres as performed with two different perfusion chambres for double sucrose gap. Pflugers Arch. 1976; 363(1):87-95. DOI: 10.1007/BF00587407. View