Cortisol Decreases the Concentration of Translatable Type-I Procollagen MRNA Species in the Developing Chick-embryo Calvaria

Overview

Journal Biochem J

Specialty Biochemistry

Date 1981 Sep 15

PMID 7326020

Citations 7

Authors

J Oikarinen

L Ryhanen

Affiliations

Soon will be listed here.

Abstract

The calvarial mRNA species of chick embryos were translated in the rabbit reticulocyte-lysate cell-free translation system. The amount of procollagen type-I mRNA species was determined by digestion with bacterial collagenase and by fluorography of the cell-free translation products. Administration of cortisol resulted in a specific decrease in the cellular concentration of translatable procollagen type-I mRNA species in the calvaria of developing chick embryos. There was a lag period of up to 12 h before the response, which was dose-dependent. The data suggest that the decrease in amounts of procollagen mRNA species is the main reason for the lower amount of tissue collagen after topical or systemic administration of glucocorticoids, although other factors may contribute to the response.

Citing Articles

Glucocorticoids stimulate collagen and noncollagen protein synthesis in cultured vascular smooth muscle cells.

Leitman D, Benson S, Johnson L J Cell Biol. 1984; 98(2):541-9.

PMID: 6693495 PMC: 2113086. DOI: 10.1083/jcb.98.2.541.

Altered steady-state ratio of type I/III procollagen mRNAs correlates with selectively increased type I procollagen biosynthesis in cultured keloid fibroblasts.

Uitto J, Perejda A, Abergel R, Chu M, Ramirez F Proc Natl Acad Sci U S A. 1985; 82(17):5935-9.

PMID: 3862108 PMC: 390668. DOI: 10.1073/pnas.82.17.5935.

Dexamethasone modulates the metabolism of type IV collagen and fibronectin in human basement-membrane-forming fibrosarcoma (HT-1080) cells.

Oikarinen A, Salo T, Ala-Kokko L, Tryggvason K Biochem J. 1987; 245(1):235-41.

PMID: 3663150 PMC: 1148105. DOI: 10.1042/bj2450235.

Gene expression of type I, III and IV collagens in hepatic fibrosis induced by dimethylnitrosamine in the rat.

Ala-Kokko L, Pihlajaniemi T, Myers J, Kivirikko K, Savolainen E Biochem J. 1987; 244(1):75-9.

PMID: 3663119 PMC: 1147955. DOI: 10.1042/bj2440075.

Transforming growth factor-beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts.

Raghow R, Postlethwaite A, Keski-Oja J, Moses H, Kang A J Clin Invest. 1987; 79(4):1285-8.

PMID: 3470308 PMC: 424335. DOI: 10.1172/JCI112950.

References

Kivirikko K, Laitinen O, Aer J, Halme J . Studies with 14C-proline on the action of cortisone on the metabolism of collagen in the rat. Biochem Pharmacol. 1965; 14(10):1445-51. DOI: 10.1016/0006-2952(65)90178-4. View

Pelham H, Jackson R . An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976; 67(1):247-56. DOI: 10.1111/j.1432-1033.1976.tb10656.x. View

Hubbard R, Matthew W, Dubowik D . Factors influencing the determination of DNA with indole. Anal Biochem. 1970; 38(1):190-201. DOI: 10.1016/0003-2697(70)90169-7. View

Peterkofsky B, Diegelmann R . Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971; 10(6):988-94. DOI: 10.1021/bi00782a009. View

Hallahan C, Young D, Munck A . Time course of early events in the action of glucocorticoids on rat thymus cells in vitro. Synthesis and turnover of a hypothetical cortisol-induced protein inhibition of glucose metabolism and of a presumed ribonucleic acid. J Biol Chem. 1973; 248(8):2922-7. View

Thompson E, Lippman M . Mechanism of action of glucocorticoids. Metabolism. 1974; 23(2):159-202. DOI: 10.1016/0026-0495(74)90113-9. View

Bonner W, Laskey R . A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974; 46(1):83-8. DOI: 10.1111/j.1432-1033.1974.tb03599.x. View

Manthorpe R, Helin G, Kofod B, Lorenzen I . Effects of glucocorticoid on connective tissue of aorta and skin in rabbits. Biochemical studies on collagen, glycosaminoglycans, DNA and RNA. Acta Endocrinol (Copenh). 1974; 77(2):310-24. DOI: 10.1530/acta.0.0770310. View

GOSPODAROWICZ D, Moran J . Stimulation of division of sparse and confluent 3T3 cell populations by a fibroblast growth factor, dexamethasone, and insulin. Proc Natl Acad Sci U S A. 1974; 71(11):4584-8. PMC: 433932. DOI: 10.1073/pnas.71.11.4584. View

10.

Cutroneo K, Stassen F, Cardinale G . Anti-inflammatory steroids and collagen metabolism: glucocorticoid-mediated decrease of prolyl hydroxylase. Mol Pharmacol. 1975; 11(1):44-51. View

11.

Laskey R, Mills A . Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975; 56(2):335-41. DOI: 10.1111/j.1432-1033.1975.tb02238.x. View

12.

Murota S, Koshihara Y, Tsurufuji S . Effects of cortisol and tetrahydrocortisol on the cloned fibroblast derived from rat carrageenin granuloma. Biochem Pharmacol. 1976; 25(9):1107-13. DOI: 10.1016/0006-2952(76)90505-0. View

13.

Oikarinen A . Effect of betamethasone-17-valerate on synthesis of collagen and prolyl hydroxylase activity in chick embryo tendon cells. Biochem Pharmacol. 1977; 26(9):875-9. DOI: 10.1016/0006-2952(77)90403-8. View

14.

Oikarinen A . Effect of cortisol acetate on collagen biosynthesis and on the activities of prolyl hydroxylase, lysyl hydroxylase, collagen galactosyltransferase and collagen glucosyltransferase in chick-embryo tendon cells. Biochem J. 1977; 164(3):533-9. PMC: 1164829. DOI: 10.1042/bj1640533. View

15.

Martial J, Seeburg P, Guenzi D, Goodman H, Baxter J . Regulation of growth hormone gene expression: synergistic effects of thyroid and glucocorticoid hormones. Proc Natl Acad Sci U S A. 1977; 74(10):4293-5. PMC: 431926. DOI: 10.1073/pnas.74.10.4293. View

16.

Kruse N, Rowe D, Fujimoto W, Bornstein P . Inhibitory effects of glucocorticoids on collagen synthesis by mouse sponge granulomas and granuloma fibroblasts in culture. Biochim Biophys Acta. 1978; 540(1):101-16. DOI: 10.1016/0304-4165(78)90439-7. View

17.

Saarni H, Tammi M . Time and concentration dependence of the action of cortisol on fibroblasts in vitro. Biochim Biophys Acta. 1978; 540(1):117-26. DOI: 10.1016/0304-4165(78)90440-3. View

18.

Rowe D, Moen R, Davidson J, Byers P, Bornstein P, Palmiter R . Correlation of procollagen mRNA levels in normal and transformed chick embryo fibroblasts with different rates of procollagen synthesis. Biochemistry. 1978; 17(9):1581-90. DOI: 10.1021/bi00602a001. View

19.

Monson J, Goodman H . Translation of chick calvarial procollagen messenger RNA'S by a messenger RNA dependent reticulocyte lysate. Biochemistry. 1978; 17(24):5122-8. DOI: 10.1021/bi00617a008. View

20.

McNelis B, Cutroneo K . A selective decrease of collagen peptide synthesis by dermal polysomes isolated from glucocorticoid-treated newborn rats. Mol Pharmacol. 1978; 14(6):1167-75. View