The Interaction of Potassium Ions and ATP on the Sodium Pump of Resealed Red Cell Ghosts

Overview

Journal J Physiol

Specialty Physiology

Date 1981 Jan 1

PMID 7320919

Citations 10

Authors

D A Eisner

D E RICHARDS

Affiliations

Soon will be listed here.

Abstract

1. Ouabain-sensitive K or Rb influx was measure into ghosts resealed to contain ATP concentrations of 1 micrometers-3 mM and no K. 2. Increasing ATP from 1 to 100 micro M, at saturation external K, increased K influx about twentyfold while have no effect on the ratio of ouabain-sensitive K influx to ouabain-sensitive ATPase activity. 3. Increasing external K decreased the apparent affinity for ATP. Similarly increasing ATP decreased the apparent affinity for external K. 4. The K influx can be empirically described as: influx = VmaxK2/(K + Kapp)2. Increasing ATP increased Vmax and (Kapp)2 by the same amount. 5. These results are consistent with a consecutive model for the Na pump in which an ATP-dependent reaction follows a K-activated dephosphorylation.

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On the functional use of the membrane compartmentalized pool of ATP by the Na+ and Ca++ pumps in human red blood cell ghosts.

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