The Effect of Membrane Phospholipid Acyl-chain Composition on the Activity of Brain-beta-N-acetyl-D-glucosaminidase

Overview

Journal Biochem J

Specialty Biochemistry

Date 1981 May 1

PMID 7316957

Citations 3

Authors

A Orlacchio

C Maffei

L Binaglia

G Porcellati

Affiliations

Soon will be listed here.

Abstract

The phospholipid acyl-chain dependence of the membrane-bound lysosomal beta-N-acetyl-D-glucosaminidase has been examined on control membranes from rat brain primary cell cultures and on membrane modified by culturing the cells in media supplemented with polyunsaturated fatty acids. The relationship between beta-N-acetyl-D-glucosaminidase activity and the membrane phospholipid acyl-chain composition has been evaluated. An increase in the unsaturation level of phosphatidyl ethanolamines and phosphatidylcholines, the most abundant phospholipids in this membrane fraction, is related to the rate of the enzymic reaction. The Arrhenius plot of the enzyme activity in modified membranes shows break-temperatures, starting from approximately 15 degrees C. The apparent activation energy below and above the break-temperature is not correlated with phospholipid acyl-chain unsaturation.

Citing Articles

Developmental quotient at 24 months and fatty acid composition of diet in early infancy: a follow up study.

Agostoni C, Trojan S, Bellu R, Riva E, Bruzzese M, Giovannini M Arch Dis Child. 1997; 76(5):421-4.

PMID: 9196357 PMC: 1717175. DOI: 10.1136/adc.76.5.421.

Effect of diet on the fatty acid composition of the major phospholipids of infant cerebral cortex.

FARQUHARSON J, Jamieson E, Abbasi K, Patrick W, Logan R, Cockburn F Arch Dis Child. 1995; 72(3):198-203.

PMID: 7741563 PMC: 1511055. DOI: 10.1136/adc.72.3.198.

Chromatofocusing coupled with automated assay for beta-hexosaminidase isoenzymes in GM2 gangliosidosis.

Orlacchio A, Maffei C, Emiliani C, Coppa G, Felici L Experientia. 1985; 41(4):525-7.

PMID: 3157597 DOI: 10.1007/BF01966184.

References

Koshland Jr D, NEMETHY G, Filmer D . Comparison of experimental binding data and theoretical models in proteins containing subunits. Biochemistry. 1966; 5(1):365-85. DOI: 10.1021/bi00865a047. View

Munn E, Greenwood C, Orlacchio A, Porcellati G . Unusual molecular weight forms of N-acetyl-beta-D-hexosaminidase from intestinal mucosa of newborn pigs. Int J Biochem. 1979; 10(12):1045-52. DOI: 10.1016/0020-711x(79)90087-9. View

Sandhoff K, Andreae U, JATZKEWITZ H . Deficient hexosaminidase activity in an exceptional case of Tay-Sachs disease with additional storage of kidney globoside in visceral organs. Pathol Eur. 1968; 3(2):278-85. View

Okada S, OBrien J . Tay-Sachs disease: generalized absence of a beta-D-N-acetylhexosaminidase component. Science. 1969; 165(3894):698-700. DOI: 10.1126/science.165.3894.698. View

Sun G . Effects of a fatty acid deficiency on lipids of whole brain, microsomes, and myelin in the rat. J Lipid Res. 1972; 13(1):56-62. View

Galli C, Trzeciak H, Paoletti R . Effects of essential fatty acid deficiency on myelin and various subcellular structures in rat brain. J Neurochem. 1972; 19(8):1863-7. DOI: 10.1111/j.1471-4159.1972.tb01475.x. View

Yavin E, MENKES J . The culture of dissociated cells from rat cerebral cortex. J Cell Biol. 1973; 57(1):232-7. PMC: 2108943. DOI: 10.1083/jcb.57.1.232. View

Coleman R . Membrane-bound enzymes and membrane ultrastructure. Biochim Biophys Acta. 1973; 300(1):1-30. DOI: 10.1016/0304-4157(73)90010-5. View

Sun G, Sun A . Synaptosomal plasma membranes: acyl group composition of phosphoglycerides and (Na+ plus K+)-ATPase activity during fatty acid deficiency. J Neurochem. 1974; 22(1):15-8. DOI: 10.1111/j.1471-4159.1974.tb12173.x. View

10.

Bernsohn J, Spitz F . Linoleic- and linolenic acid dependency of some brain membrane-bound enzymes after lipid deprivation in rats. Biochem Biophys Res Commun. 1974; 57(1):293-8. DOI: 10.1016/s0006-291x(74)80389-x. View

11.

Montamat E, Aeberhard E, de Bronia D . Effect of essential fatty acid deficiency on brain lipids of developing rats. Acta Physiol Lat Am. 1974; 24(1):63-4. View

12.

Farias R, Bloj B, Morero R, Sineriz F, TRUCCO R . Regulation of allosteric membrane-bound enzymes through changes in membrane lipid compostition. Biochim Biophys Acta. 1975; 415(2):231-51. DOI: 10.1016/0304-4157(75)90003-9. View

13.

Chapman D . Phase transitions and fluidity characteristics of lipids and cell membranes. Q Rev Biophys. 1975; 8(2):185-235. DOI: 10.1017/s0033583500001797. View

14.

Flower R, Blackwell G . The importance of phospholipase-A2 in prostaglandin biosynthesis. Biochem Pharmacol. 1976; 25(3):285-91. DOI: 10.1016/0006-2952(76)90216-1. View

15.

Marinkovic D, Marinkovic J . Isolation and properties of alpha-D-mannosidase from human kidney. Biochem J. 1976; 155(2):217-23. PMC: 1172826. DOI: 10.1042/bj1550217. View

16.

Srivastava S, Wiktorowicz J, Awasthi Y . Interrelationship of hexosaminidases A and B: conformation of the common and the unique subunit theory. Proc Natl Acad Sci U S A. 1976; 73(8):2833-7. PMC: 430766. DOI: 10.1073/pnas.73.8.2833. View

17.

Van Kessel W, Hax W, Demel R, de Gier J . High performance liquid chromatographic separation and direct ultraviolet detection of phospholipids. Biochim Biophys Acta. 1977; 486(3):524-30. DOI: 10.1016/0005-2760(77)90102-3. View

18.

Ruth R, Weglicki W . The temperature-dependence of the loss of latency of lysosomal enzymes. Biochem J. 1978; 172(1):163-73. PMC: 1185675. DOI: 10.1042/bj1720163. View

19.

Robert J, Rebel G, Mandel P . Utilization of polyunsaturated fatty acid supplements by cultured neuroblastoma cells. J Neurochem. 1978; 30(3):543-8. DOI: 10.1111/j.1471-4159.1978.tb07807.x. View

20.

Orlacchio A, Flagiello A, Mozzi R, Porcellati G . The effect of prostaglandins F2alpha and E2 on rat brain N-acetyl-beta-D-glucosaminidase in vitro. Pharmacol Res Commun. 1978; 10(9):839-50. DOI: 10.1016/s0031-6989(78)80103-9. View