The Cytochromes in Microsomal Fractions of Germinating Mung Beans

Overview

Journal Biochem J

Specialty Biochemistry

Date 1981 Mar 15

PMID 7306021

Citations 16

Authors

G A Hendry

J D HOUGHTON

O T Jones

Affiliations

Soon will be listed here.

Abstract

Detailed studies of microsomal cytochromes from mung-bean radicles showed the presence of cytochrome P-420, particularly in dark-grown seedlings, accompanied by smaller quantities of cytochrome P-450. Similar proportions of cytochrome P-420 to cytochrome P-450 were found spectrophotometrically in vivo with whole radicles and hypocotyls. Assayed in vitro, maximum concentrations of both cytochromes were attained after 4 days of growth, before undergoing rapid degradation. Illumination of seedlings stabilized cytochrome P-450 and decreased the amount of cytochrome P-420. Three b cytochromes were present in the microsomal fraction, namely cytochromes b-562.5 (Em + 105 +/- 23 mV), b-560.5 (Em + 49 +/- 13 mV) and b5 (Em - 45 +/- 14 mV), all at pH 7.0. Of the b cytochromes, cytochrome b5 alone undergoes a rapid degradation after day 4, Changes in cytochrome b concentrations were confined to the microsomal fraction: mitochondrial b cytochrome concentrations were unaltered with age. Protohaem degradation (of exogenous methaemalbumin) was detected in microsomal fractions of mung beans. The rates of degradation were highest in extracts of young tissue and declined after day 4. The degradation mechanism and products did not resemble those of mammalian haem oxygenase.

Citing Articles

Cytochromes b561: ascorbate-mediated trans-membrane electron transport.

Asard H, Barbaro R, Trost P, Berczi A Antioxid Redox Signal. 2012; 19(9):1026-35.

PMID: 23249217 PMC: 3763232. DOI: 10.1089/ars.2012.5065.

Development of Endoplasmic Reticulum and Glyoxysomal Membrane Redox Activities during Castor Bean Germination.

Alani A, Luster D, Donaldson R Plant Physiol. 1990; 94(4):1842-8.

PMID: 16667925 PMC: 1077462. DOI: 10.1104/pp.94.4.1842.

b-Type Cytochromes in Higher Plant Plasma Membranes.

Asard H, Venken M, Caubergs R, Reijnders W, Oltmann F, De Greef J Plant Physiol. 1989; 90(3):1077-83.

PMID: 16666854 PMC: 1061846. DOI: 10.1104/pp.90.3.1077.

Features of Rhodobacter sphaeroides CcmFH.

Rios-Velazquez C, Coller R, Donohue T J Bacteriol. 2003; 185(2):422-31.

PMID: 12511487 PMC: 145331. DOI: 10.1128/JB.185.2.422-431.2003.

Higher-plant plasma membrane cytochrome b561: a protein in search of a function.

Asard H, Kapila J, Verelst W, Berczi A Protoplasma. 2001; 217(1-3):77-93.

PMID: 11732342 DOI: 10.1007/BF01289417.

References

Rich P, Bendall D . Cytochrome components of plant microsomes. Eur J Biochem. 1975; 55(2):333-41. DOI: 10.1111/j.1432-1033.1975.tb02167.x. View

HOLTZMAN D, Desautel M . Microsomal cytochromes in the immature and adult rat brain. J Neurochem. 1980; 34(6):1535-7. DOI: 10.1111/j.1471-4159.1980.tb11237.x. View

Rich P, Lamb C . Biophysical and enzymological studies upon the interaction of trans-cinnamic acid with higher plant microsomal cytochromes P-450. Eur J Biochem. 1977; 72(2):353-60. DOI: 10.1111/j.1432-1033.1977.tb11259.x. View

Castelfranco P, Jones O . Protoheme turnover and chlorophyll synthesis in greening barley tissue. Plant Physiol. 1975; 55(3):485-90. PMC: 541643. DOI: 10.1104/pp.55.3.485. View

Matsubara T, Koike M, Touchi A, Tochino Y, SUGENO K . Quantitative determination of cytochrome P-450 in rat liver homogenate. Anal Biochem. 1976; 75(2):596-603. DOI: 10.1016/0003-2697(76)90114-7. View

Shichi H, Hackett D . Studies on the b-type cytochromes from mung bean seedlings. II. Some properties of cytochromes b-555 and b-561. J Biol Chem. 1962; 237:2959-64. View

TENHUNEN R, MARVER H, Schmid R . The enzymatic conversion of heme to bilirubin by microsomal heme oxygenase. Proc Natl Acad Sci U S A. 1968; 61(2):748-55. PMC: 225223. DOI: 10.1073/pnas.61.2.748. View

Omura T, Sato R . THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE. J Biol Chem. 1964; 239:2370-8. View

Ishimaru A, Yamazaki I . The carbon monoxide-binding hemoprotein reducible by hydrogen peroxide in microsomal fractions of pea seeds. J Biol Chem. 1977; 252(1):199-204. View

10.

Potts J, Weklych R, Conn E, Rowell J . The 4-hydroxylation of cinnamic acid by sorghum microsomes and the requirement for cytochrome P-450. J Biol Chem. 1974; 249(16):5019-26. View

11.

Murphy P, West C . The role of mixed function oxidases in kaurene metabolism in Echinocystis macrocarpa Greene endosperm. Arch Biochem Biophys. 1969; 133(2):395-407. DOI: 10.1016/0003-9861(69)90468-8. View

12.

Dutton P . Redox potentiometry: determination of midpoint potentials of oxidation-reduction components of biological electron-transfer systems. Methods Enzymol. 1978; 54:411-35. DOI: 10.1016/s0076-6879(78)54026-3. View

13.

Rich P, Cammack R, Bendall D . Electron paramagnetic resonance studies of cytochrome P-450 in plant microsomes. Eur J Biochem. 1975; 59(1):281-6. DOI: 10.1111/j.1432-1033.1975.tb02453.x. View

14.

Meehan T, Coscia C . Hydroxylation of geraniol and nerol by a monooxygenase from Vinca rosea. Biochem Biophys Res Commun. 1973; 53(4):1043-8. DOI: 10.1016/0006-291x(73)90570-6. View

15.

Rich P, Bendall D . The redox potentials of the b-type cytochromes of higher plant chloroplasts. Biochim Biophys Acta. 1980; 591(1):153-61. DOI: 10.1016/0005-2728(80)90229-7. View

16.

Lodola A, Hendry G, Jones O . Haem oxygenase: a reappraisal of the stoicheiometry. FEBS Lett. 1979; 104(1):45-50. DOI: 10.1016/0014-5793(79)81082-0. View

17.

Granick S, Beale S . Hemes, chlorophylls, and related compounds: biosynthesis and metabolic regulation. Adv Enzymol Relat Areas Mol Biol. 1978; 46:33-203. DOI: 10.1002/9780470122914.ch2. View

18.

Salaun J, Benveniste I, Reichhart D, Durst F . A microsomal (cytochrome P-450)-linked lauric-acid-monooxygenase from aged Jerusalem-artichoke-tuber tissues. Eur J Biochem. 1978; 90(1):155-9. DOI: 10.1111/j.1432-1033.1978.tb12586.x. View

19.

Abdelkader A, Cherif A, Demandre C, Mazliak P . The oleyl-coenzyme-A desaturase of potato tubers. Enzymatic properties, intracellular localization and induction during "aging" of tuber slices. Eur J Biochem. 1973; 32(1):155-65. DOI: 10.1111/j.1432-1033.1973.tb02592.x. View

20.

Siekevitz P . Electron transport systems in microsomes. Origin and functional nature of microsomes. Fed Proc. 1965; 24(5):1153-5. View