Relative Synthesis of Cardiac Contractile Proteins. Evidence for Synthesis from the Same Precursor Pool

Overview

Journal Biochem J

Specialty Biochemistry

Date 1981 Mar 15

PMID 7306015

Authors

C D Evans

S S SCHREIBER

M ORATZ

M A Rothschild

Affiliations

Soon will be listed here.

Abstract

The relative molar synthesis of cardiac contractile proteins has been measured in the perfused heart under control haemodynamic conditions. This synthesis, of myosin heavy chains, individual light chains (1 and 2), actin and tropomyosin, was determined from isolated guinea-pig hearts perfused for 3h simultaneously with constant specific radioactivities and concentrations of [3H]lysine and [3H]phenylalanine. The data strongly suggest that all of the proteins studied were synthesized from the same precursor pools of lysine and phenylalanine, since the ratio of the specific activities of the two labels was the same in all of the proteins. Measurement of molar synthesis of each contractile protein was the same with either labelled amino acid. Under control haemodynamic-perfusion conditions, the relative molar synthesis of the contractile proteins was actin greater than heavy chains greater than light chain 2 greater than light chain 1 greater than tropomyosin.

References

Morkin E . Stimulation of cardiac myosin adenosine triphosphatase in thyrotoxicosis. Circ Res. 1979; 44(1):1-7. DOI: 10.1161/01.res.44.1.1. View

Reddy Y, HONIG C . Ca 2+ -binding and Ca 2+ -sensitizing functions of cardiac native tropomyosin, troponin, and tropomyosin. Biochim Biophys Acta. 1972; 275(3):453-63. DOI: 10.1016/0005-2728(72)90226-5. View

Fern E, Garlick P . Compartmentation of albumin and ferritin synthesis in rat liver in vivo. Biochem J. 1976; 156(1):189-92. PMC: 1163732. DOI: 10.1042/bj1560189. View

Zak R, Martin A, Prior G, Rabinowitz M . Comparison of turnover of several myofibrillar proteins and critical evaluation of double isotope method. J Biol Chem. 1977; 252(10):3430-5. View

Tsukui R, Ebashi S . Cardiac troponin. J Biochem. 1973; 73(5):1119-21. DOI: 10.1093/oxfordjournals.jbchem.a130168. View

Katz A, CARSTEN M . ACTIN FROM HEART MUSCLE: STUDIES ON AMINO ACID COMPOSITION. Circ Res. 1963; 13:474-7. DOI: 10.1161/01.res.13.5.474. View

Lagrange B, Low R . Turnover of myosin heavy and light chains in cultured embryonic chick cardiac and skeletal muscle. Dev Biol. 1976; 54(2):214-29. DOI: 10.1016/0012-1606(76)90300-6. View

Fenner C, Valentine R, Mason D, Wikman-Coffelt J . Purification and characterization of cardiac tropomyosins. Prep Biochem. 1975; 5(3):189-97. DOI: 10.1080/00327487508061571. View

SCHREIBER S, Rothschild M, Evans C, Reff F, ORATZ M . The effect of pressure or flow stress on right ventricular protein synthesis in the face of constant and restricted coronary perfusion. J Clin Invest. 1975; 55(1):1-11. PMC: 301711. DOI: 10.1172/JCI107899. View

10.

HUTZLER J, Odievre M, DANCIS J . Analysis for lysine, arginine, histidine, and tyrosine in biological fluids. Anal Biochem. 1967; 19(3):529-41. DOI: 10.1016/0003-2697(67)90243-6. View

11.

Raszkowski R, WELTY J, Peterson M . The amino acid composition of actin and myosin and Ca2+-activated myosin adenosine triphosphatase in chronic canine congestive heart failure. Circ Res. 1977; 40(2):191-8. DOI: 10.1161/01.res.40.2.191. View

12.

CARSTEN M, Katz A . ACTIN: A COMPARATIVE STUDY. Biochim Biophys Acta. 1964; 90:534-41. DOI: 10.1016/0304-4165(64)90232-6. View

13.

Wikman-Coffelt J, Zelis R, Fenner C, Mason D . Myosin chains of myocardial tissue. I. Purification and immunological properties of myosin heavy chains. Biochem Biophys Res Commun. 1973; 51(4):1097-104. DOI: 10.1016/0006-291x(73)90040-5. View

14.

Ilan J, Singer M . Sampling of the leucine pool from the growing peptide chain: difference in leucine specific activity of peptidyl-transfer RNA from free and membrane-bound polysomes. J Mol Biol. 1975; 91(1):39-51. DOI: 10.1016/0022-2836(75)90370-8. View

15.

Harding G, Buckwold F, Marrie T, Thompson L, Light R, Ronald A . Prophylaxis of recurrent urinary tract infection in female patients. Efficacy of low-dose, thrice-weekly therapy with trimethoprim-sulfamethoxazole. JAMA. 1979; 242(18):1975-7. View

16.

Hider R, Fern E, London D . Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis. Biochem J. 1971; 121(5):817-27. PMC: 1176670. DOI: 10.1042/bj1210817. View

17.

SCHREIBER S, ORATZ M, Rothschild M . Protein synthesis in the overloaded mammalian heart. Am J Physiol. 1966; 211(2):314-8. DOI: 10.1152/ajplegacy.1966.211.2.314. View

18.

Sender P . Muscle fibrils: Solubilization and gel electrophoresis. FEBS Lett. 1971; 17(1):106-110. DOI: 10.1016/0014-5793(71)80575-6. View

19.

Cummins P, PERRY S . Chemical and immunochemical characteristics of tropomyosins from striated and smooth muscle. Biochem J. 1974; 141(1):43-9. PMC: 1168047. DOI: 10.1042/bj1410043. View

20.

Sanford C, Griffin E, Wildenthal K . Synthesis and degradation of myocardial protein during the development and regression of thyroxine-induced cardiac hypertrophy in rats. Circ Res. 1978; 43(5):688-94. DOI: 10.1161/01.res.43.5.688. View