Studies of Pyruvate-water Isotope Exchange Catalysed by Erythrocytes and Proteins

Overview

Journal Biochem J

Specialty Biochemistry

Date 1981 Feb 1

PMID 7305939

Citations 4

Authors

R J Simpson

K M Brindle

F F Brown

I D Campbell

D L Foxall

Affiliations

Soon will be listed here.

Abstract

Erythrocyte suspensions in buffer made with 2H2O catalyse the exchange of pyruvate protons. This process can be easly observed by spin-echo proton magnetic resonance. The dominant exchange process is shown to be due to the formation of Schiff-base links between pyruvate and amino groups of haemoglobin. Other proteins with free alpha-amino groups also catalyse the exchange. The pH*-dependence of the exchange rate due to hen-egg-white-lysozyme reflects the dissociation of the alpha-amino group.

Citing Articles

Deuterium Metabolic Imaging of the Brain Using 2-Deoxy-2-[H]-d-glucose: A Non-ionizing [F]FDG Alternative.

Gao X, Qiao K, Wilson D, Chaumeil M, Gordon J JACS Au. 2025; 5(2):571-577.

PMID: 40017751 PMC: 11862922. DOI: 10.1021/jacsau.4c00888.

Effects of deuteration on transamination and oxidation of hyperpolarized C-Pyruvate in the isolated heart.

Funk A, Wen X, Hever T, Maptue N, Khemtong C, Sherry A J Magn Reson. 2019; 301:102-108.

PMID: 30861456 PMC: 6666394. DOI: 10.1016/j.jmr.2019.03.003.

13C and 1H nuclear magnetic resonance study of glycogen futile cycling in strains of the genus Fibrobacter.

Matheron C, Delort A, Gaudet G, Forano E, Liptaj T Appl Environ Microbiol. 2002; 64(1):74-81.

PMID: 12033219 PMC: 124674. DOI: 10.1128/AEM.64.1.74-81.1998.

A 1H n.m.r. study of isotope exchange catalysed by glycolytic enzymes in the human erythrocyte.

Brindle K, Brown F, Campbell I, Foxall D, Simpson R Biochem J. 1982; 202(3):589-602.

PMID: 7092833 PMC: 1158152. DOI: 10.1042/bj2020589.

A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells.

Simpson R, Brindle K, Brown F, Campbell I, Foxall D Biochem J. 1982; 202(3):573-9.

PMID: 7092831 PMC: 1158150. DOI: 10.1042/bj2020573.

References

Freedman R, Radda G . The reaction of 2,4,6-trinitrobenzenesulphonic acid with amino acids, Peptides and proteins. Biochem J. 1968; 108(3):383-91. PMC: 1198823. DOI: 10.1042/bj1080383. View

Shinoda T . THE APPARENT HIGH REACTIVITY OF SOME AMINO GROUPS OF NATIVE HEMOGLOBIN. Biochim Biophys Acta. 1965; 97:382-4. DOI: 10.1016/0304-4165(65)90117-0. View

Nowak T, Mildvan A . Stereoselective interactions of phosphoenolpyruvate analogues with phosphoenolpyruvate-utilizing enzymes. J Biol Chem. 1970; 245(22):6057-64. View

Cerami A, Manning J . Potassium cyanate as an inhibitor of the sickling of erythrocytes in vitro. Proc Natl Acad Sci U S A. 1971; 68(6):1180-3. PMC: 389147. DOI: 10.1073/pnas.68.6.1180. View

Arnone A . X-ray diffraction study of binding of 2,3-diphosphoglycerate to human deoxyhaemoglobin. Nature. 1972; 237(5351):146-9. DOI: 10.1038/237146a0. View

Garner M, Bogardt Jr R, Gurd F . Determination of the pK values for the alpha-amino groups of human hemoglobin. J Biol Chem. 1975; 250(12):4398-404. View

Katz J, ROGNSTAD R . Futile cycles in the metabolism of glucose. Curr Top Cell Regul. 1976; 10:237-89. DOI: 10.1016/b978-0-12-152810-2.50013-9. View

ROGNSTAD R, Wals P . The metabolism of l-[3-3h]lactate by isolated hamster liver cells. Biochim Biophys Acta. 1976; 437(1):16-21. DOI: 10.1016/0304-4165(76)90343-3. View

Halestrap A . Transport of pyruvate nad lactate into human erythrocytes. Evidence for the involvement of the chloride carrier and a chloride-independent carrier. Biochem J. 1976; 156(2):193-207. PMC: 1163737. DOI: 10.1042/bj1560193. View

10.

Meloche H, Monti C, Cleland W . Magnitude of the equilibrium isotope effect on carbon-tritium bond synthesis. Biochim Biophys Acta. 1977; 480(2):517-9. DOI: 10.1016/0005-2744(77)90047-x. View

11.

Brown F, Campbell I, Kuchel P, Rabenstein D . Human erythrocyte metabolism studies by 1H spin echo NMR. FEBS Lett. 1977; 82(1):12-6. DOI: 10.1016/0014-5793(77)80875-2. View

12.

BUNN H, GABBAY K, Gallop P . The glycosylation of hemoglobin: relevance to diabetes mellitus. Science. 1978; 200(4337):21-7. DOI: 10.1126/science.635569. View

13.

Campbell I, Dobson C . The application of high resolution nuclear magnetic resonance to biological systems. Methods Biochem Anal. 1979; 25:1-133. DOI: 10.1002/9780470110454.ch1. View

14.

ROGNSTAD R . Pyruvate cycling involving possible oxaloacetate decarboxylase activity. Biochim Biophys Acta. 1979; 586(2):242-9. DOI: 10.1016/0304-4165(79)90096-5. View

15.

Brindle K, Brown F, Campbell I, Grathwohl C, Kuchel P . Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport. Biochem J. 1979; 180(1):37-44. PMC: 1161016. DOI: 10.1042/bj1800037. View

16.

Acharya A, Manning J . Reactivity of the amino groups of carbonmonoxyhemoglobin S with glyceraldehyde. J Biol Chem. 1980; 255(4):1406-12. View

17.

Rose I . Studies on the enolization of pyruvate by pyruvate kinase. J Biol Chem. 1960; 235:1170-7. View

18.

KOSICKI G . Oxaloacetate decarboxylase from cod. Catalysis of hydrogen-deuterium exchange in pyruvate. Biochemistry. 1968; 7(12):4310-4. DOI: 10.1021/bi00852a024. View