Deviations from Michaelis-Menten Kinetics. Computation of the Probabilities of Obtaining Complex Curves from Simple Kinetic Schemes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1981 Jan 1

PMID 7305929

Citations 2

Authors

P B McGinlay

R Woolfson

W G Bardsley

Affiliations

Soon will be listed here.

Abstract

1. It is possible to calculate the intrinsic probability associated with any curve shape that is allowed for rational functions of given degree when the coefficients are independent or dependent random variables with known probability distributions. 2. Computations of such probabilities are described when the coefficients of the rational function are generated according to several probability distribution functions and in particular when rate constants are varied randomly for several simple model mechanisms. 3. It is concluded that each molecular mechanism is associated with a specific set of curve-shape probabilities, and this could be of value in discriminating between model mechanisms. 4. It is shown how a computer program can be used to estimate the probability of new complexities such as extra inflexions and turning points as the degree of rate equations increases. 5. The probability of 3 : 3 rate equations giving 2 : 2 curve shapes is discussed for unrestricted coefficients and also for the substrate-modifier mechanisms. 6. The probability associated with the numerical values of coefficients in rate equations is also calculated for this mechanism, and a possible method for determining the approximate magnitude of product-release steps is given. 7. The computer programs used in the computations have been deposited as Supplement SUP 50113 (21 pages) with the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem, J. (1978) 169, 5.

Citing Articles

The probability that complex enzyme kinetic curves can be caused by activators of inhibitors.

Bardsley W, Indge K Biochem J. 1981; 195(3):589-601.

PMID: 7316974 PMC: 1162930. DOI: 10.1042/bj1950589.

Use of the F test for determining the degree of enzyme-kinetic and ligand-binding data. A Monte Carlo simulation study.

Burguillo F, Wright A, Bardsley W Biochem J. 1983; 211(1):23-34.

PMID: 6870821 PMC: 1154325. DOI: 10.1042/bj2110023.

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