Glycerophospholipid Metabolism in Neuronal and Glial Cell-enriched Fractions

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1981 Jul 1

PMID 7301041

Citations 1

Authors

G Arienti

G Goracci

G Porcellati

Affiliations

Soon will be listed here.

Abstract

The metabolism of phospholipids in separated glial and neuronal cells has been reviewed in this paper. Lipids are more abundant in glia; on the other hand, in vivo experiments performed with labeled precursors have indicated that lipid turnover is faster in neurons (with the possible exception of oligodendroglia). Biosynthetic and catabolic enzymes of lipid metabolism have been studied in separated cells (mainly in neurons and astroglia) and have been shown to be almost always more active in neurons. Also base exchange is probably more active in these cells. Therefore the results of in vitro and in vivo experiments indicate that neurons are more active than astroglia in metabolizing glycerophospholipids.

Citing Articles

Cell-Type-Specific Metabolic Profiling Achieved by Combining Desorption Electrospray Ionization Mass Spectrometry Imaging and Immunofluorescence Staining.

Yan X, Zhao X, Zhou Z, McKay A, Brunet A, Zare R Anal Chem. 2020; 92(19):13281-13289.

PMID: 32880432 PMC: 8782277. DOI: 10.1021/acs.analchem.0c02519.

References

Dorman R, Toews A, Horrocks L . Plasmalogenase activities in neuronal perikarya, astroglia, and oligodendroglia isolated from bovine brain. J Lipid Res. 1977; 18(1):115-7. View

Huttner W, Meyermann R, Neuhoff V, Althaus H . Neurochemical and morphological studies of bulk isolated rat brain cells. II. Preparation of viable cerebral neurons which retain synaptic complexes. Brain Res. 1979; 171(2):225-37. DOI: 10.1016/0006-8993(79)90329-9. View

Goracci G, Porcellati G, Woelk H . Subcellular localization and distribution of phospholipases A in liver and brain tissue. Adv Prostaglandin Thromboxane Res. 1978; 3:55-67. View

Porcellati G, Arienti G, Pirotta M, Giorgini D . Base-exchange reactions for the synthesis of phospholipids in nervous tissue: the incorporation of serine and ethanolamine into the phospholipids of isolated brain microsomes. J Neurochem. 1971; 18(8):1395-417. DOI: 10.1111/j.1471-4159.1971.tb00004.x. View

Rose S, Sinha A . Some properties of isolated neuronal cell fractions. J Neurochem. 1969; 16(9):1319-28. DOI: 10.1111/j.1471-4159.1969.tb05982.x. View

Chao S, Rumsby M . Isolation of oligodendrocytes and other cell lines from whole rat brain tissue. Biochem Soc Trans. 1977; 5(1):194-6. DOI: 10.1042/bst0050194. View

Crews F, Hirata F, Axelrod J . Identification and properties of methyltransferases that synthesize phosphatidylcholine in rat brain synaptosomes. J Neurochem. 1980; 34(6):1491-8. DOI: 10.1111/j.1471-4159.1980.tb11229.x. View

Raghavan S, Rhoads D, Kanfer J . Acid hydrolases in neuronal and glial enriched fractions of rat brain. Biochim Biophys Acta. 1972; 268(3):755-60. DOI: 10.1016/0005-2744(72)90285-9. View

Blusztajn J, Zeisel S, Wurtman R . Synthesis of lecithin (phosphatidylcholine) from phosphatidylethanolamine in bovine brain. Brain Res. 1979; 179(2):319-27. DOI: 10.1016/0006-8993(79)90447-5. View

10.

Raghavan S, Rhoads D, KANFER J . In vitro incorporation of ( 14 C)serine, ( 14 C)ethanolamine, and ( 14 C)choline into phospholipids of neuronal and glial-enriched fractions from rat brain by base exchange. J Biol Chem. 1972; 247(22):7153-6. View

11.

Johnson D, Sellinger O . Protein synthesis in neurons and glial cells of the developing rat brain: an in vivo study. J Neurochem. 1971; 18(8):1445-60. DOI: 10.1111/j.1471-4159.1971.tb00007.x. View

12.

Althaus H, Neuhoff V, Huttner W, Monzain R, Shahar A . Maintenance and regeneration in vitro of bulk-isolated cortical neurones from adult rats. Hoppe Seylers Z Physiol Chem. 1978; 359(6):773-5. DOI: 10.1515/bchm.1978.359.1.773. View

13.

Brammer M, Carey S . Incorporation of choline and inositol into phospholipids of isolated bovine oligodendrocyte perikarya. J Neurochem. 1980; 35(4):873-9. DOI: 10.1111/j.1471-4159.1980.tb07085.x. View

14.

Farooq M, Norton W . A modified procedure for isolation of astrocyte- and neuron-enriched fractions from rat brain. J Neurochem. 1978; 31(4):887-94. DOI: 10.1111/j.1471-4159.1978.tb00124.x. View

15.

Kanfer J . Base exchange reactions of the phospholipids in rat brain particles. J Lipid Res. 1972; 13(4):468-76. View

16.

Freysz L, Farooqui A, Mandel P . Lysosomal hydrolases in neuronal, astroglial, and olidodendroglial enriched fractions of rabbit and beef brain. J Lipid Res. 1979; 20(4):503-8. View

17.

Rose S . Preparation of enriched fractions from cerebral cortex containing isolated, metabolically active neuronal and glial cells. Biochem J. 1967; 102(1):33-43. PMC: 1270206. DOI: 10.1042/bj1020033. View

18.

Poduslo S, Norton W . Isolation and some chemical properties of oligodendroglia from calf brain. J Neurochem. 1972; 19(3):727-36. DOI: 10.1111/j.1471-4159.1972.tb01388.x. View

19.

Althaus H, Gebicke P, Neuhoff V, Shahar A . Primate brain nerve cells bulk isolated by the perfusion method. Naturwissenschaften. 1979; 66(2):117-8. DOI: 10.1007/BF00373512. View

20.

Binaglia L, Roberti R, Goracci G, Francescangeli E, Porcellati G . Enzymic synthesis of ethanolamine plasmalogens through ethanolaminephosphotransferase activity in neurons and glial cells of rabbit in vitro. Lipids. 1974; 9(10):738-47. DOI: 10.1007/BF02532139. View