The Chromosomal Distribution of Repetitive DNA Sequences Within the Human Beta Globin Gene Cluster

Overview

Journal Hum Genet

Specialty Genetics

Date 1981 Jan 1

PMID 7286979

Citations 1

Authors

S Malcolm

P Barton

M A Ferguson-Smith

Affiliations

Soon will be listed here.

Abstract

The chromosomal distribution and degree of repetitiveness of the sequences within the human beta globin gene cluster has been studied by in situ hybridisation. A genomic recombinant, H beta G1, which contains 15.9 thousand base pairs (kb) of DNA inserted into lambda charon 4A, was used as a template for the [3H] complementary RNA used for hybridisation. The inserted DNA contains sequences 4.7 kb to the 5' side of the delta gene and continues through the delta and beta genes to a site 2.8 kb to the 3' side of the beta gene. Two highly repetitive sequences, which are distributed evenly over all the chromosomes, have been identified within this DNA. Another somewhat less repetitive sequence has been identified between the beta and delta genes.

Citing Articles

Localization and characterization of members of a family of repetitive sequences in the goat beta globin locus.

Spence S, Young R, Garner K, Lingrel J Nucleic Acids Res. 1985; 13(6):2171-86.

PMID: 4000955 PMC: 341143. DOI: 10.1093/nar/13.6.2171.

References

Fritsch E, Lawn R, Maniatis T . Molecular cloning and characterization of the human beta-like globin gene cluster. Cell. 1980; 19(4):959-72. DOI: 10.1016/0092-8674(80)90087-2. View

Lauer J, Shen C, Maniatis T . The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions. Cell. 1980; 20(1):119-30. DOI: 10.1016/0092-8674(80)90240-8. View

de la Chapelle A, MILLER O . Report of the committee on the genetic constitution of chromosomes 10, 11, 12, X, and Y. Cytogenet Cell Genet. 1979; 25(1-4):47-58. DOI: 10.1159/000131399. View

Miklos G, John B . Heterochromatin and satellite DNA in man: properties and prospects. Am J Hum Genet. 1979; 31(3):264-80. PMC: 1685796. View

Duncan C, Biro P, Choudary P, Elder J, Wang R, Forget B . RNA polymerase III transcriptional units are interspersed among human non-alpha-globin genes. Proc Natl Acad Sci U S A. 1979; 76(10):5095-9. PMC: 413086. DOI: 10.1073/pnas.76.10.5095. View

Bazaral M, Helinski D . Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J Mol Biol. 1968; 36(2):185-94. DOI: 10.1016/0022-2836(68)90374-4. View

Lawn R, Fritsch E, Parker R, Blake G, Maniatis T . The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell. 1978; 15(4):1157-74. DOI: 10.1016/0092-8674(78)90043-0. View

Boyd C, Tolstoshev P, Schafer M, Trapnell B, Coon H, Kretschmer P . Isolation and characterization of a 15-kilobase genomic sequence coding for part of the Pro alpha 2 chain of sheep type I collagen. J Biol Chem. 1980; 255(7):3212-20. View

Britten R, Kohne D . Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968; 161(3841):529-40. DOI: 10.1126/science.161.3841.529. View

10.

Houck C, Rinehart F, Schmid C . A ubiquitous family of repeated DNA sequences in the human genome. J Mol Biol. 1979; 132(3):289-306. DOI: 10.1016/0022-2836(79)90261-4. View

11.

Maden B, Reeder R . Partial mapping of methylated sequences in Xenopus laevis ribosomal RNA by preparative hybridization to cloned fragments of ribosomal DNA. Nucleic Acids Res. 1979; 6(3):817-30. PMC: 327736. DOI: 10.1093/nar/6.3.817. View

12.

Gosden J, Mitchell A, Buckland R, Clayton R, Evans H . The location of four human satellite DNAs on human chromosomes. Exp Cell Res. 1975; 92(1):148-58. DOI: 10.1016/0014-4827(75)90648-5. View

13.

Yamamoto K, Alberts B, Benzinger R, Lawhorne L, Treiber G . Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970; 40(3):734-44. DOI: 10.1016/0042-6822(70)90218-7. View

14.

Nunberg J, Kaufman R, Chang A, Cohen S, Schimke R . Structure and genomic organization of the mouse dihydrofolate reductase gene. Cell. 1980; 19(2):355-64. DOI: 10.1016/0092-8674(80)90510-3. View

15.

KAUFMAN R, Kretschmer P, Adams J, Coon H, Anderson W, Nienhuis A . Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes. Proc Natl Acad Sci U S A. 1980; 77(7):4229-33. PMC: 349805. DOI: 10.1073/pnas.77.7.4229. View

16.

Proudfoot N . Pseudogenes. Nature. 1980; 286(5776):840-1. DOI: 10.1038/286840a0. View

17.

Young B, Hell A, BIRNIE G . A new estimate of human ribosomal gene number. Biochim Biophys Acta. 1976; 454(3):539-48. DOI: 10.1016/0005-2787(76)90279-3. View

18.

Katz L, Kingsbury D, Helinski D . Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J Bacteriol. 1973; 114(2):577-91. PMC: 251812. DOI: 10.1128/jb.114.2.577-591.1973. View

19.

Gorin M, Tilghman S . Structure of the alpha-fetoprotein gene in the mouse. Proc Natl Acad Sci U S A. 1980; 77(3):1351-5. PMC: 348492. DOI: 10.1073/pnas.77.3.1351. View

20.

Malcolm S, Williamson R, Boyd E, Ferguson-Smith M . A comparison of in situ hybridization techniques for gene localization. Cytogenet Cell Genet. 1977; 19(5):256-61. DOI: 10.1159/000130818. View