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Albumin Obscures Sex Differences in Blood Protein Patterns of Rats and Humans

Overview
Journal Biochem J
Specialty Biochemistry
Date 1980 Dec 1
PMID 7283979
Citations 3
Authors
D M Gersten
B S Khirabadi
P Kurian
R S Ledley
T Mahany
E R RAMEY
P W RAMWELL
Affiliations
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Abstract

Sex related differences in the blood protein patterns of male and female rats and humans have been studied by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. In rats, a prominent band of mol.wt. 74000--78000 is seen in females in far greater quantity than in males, castrated males or ovariectomized females. A secondary band of 100000 is seen under non-reducing conditions in female rats that is absent in males. In humans, bands of 92000 and 88000 mol.wt. appear to be variable in concentration in men although relatively constant in women. The above differences are observable only if serum albumin is removed from the samples before electrophoresis. The results suggest that each sex has its own characteristic blood protein pattern.

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References
1.
Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View
2.
Travis J, Pannell R . Selective removal of albumin from plasma by affinity chromatography. Clin Chim Acta. 1973; 49(1):49-52. DOI: 10.1016/0009-8981(73)90341-0. View
3.
Sata T, HAVEL R, KOTITE L, Kane J . New protein in human blood plasma, rich in proline, with lipid-binding properties. Proc Natl Acad Sci U S A. 1976; 73(4):1063-7. PMC: 430200. DOI: 10.1073/pnas.73.4.1063. View
4.
CASTELLI W, DOYLE J, Gordon T, HAMES C, Hjortland M, Hulley S . HDL cholesterol and other lipids in coronary heart disease. The cooperative lipoprotein phenotyping study. Circulation. 1977; 55(5):767-72. DOI: 10.1161/01.cir.55.5.767. View
5.
Kobayashi N, Takeda Y . Effects of a large dose of oestradiol on antithrombin III metabolism in male and female dogs. Eur J Clin Invest. 1977; 7(5):373-81. DOI: 10.1111/j.1365-2362.1977.tb01622.x. View