GABA Transport in the Rat Thyroid

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1981 Aug 1

PMID 7279009

Citations 3

Authors

H GEBAUER

Affiliations

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Abstract

1. The uptake of gamma-aminobutyric acid (GABA) into rat thyroid slices was studied. 2. Uptake of 14C-GABA was concentration-dependent: one unsaturable (diffusion) and two saturable components obeying Michaelis-Menten kinetics contributed to transport. 3. The kinetic constants of saturable GABA transport systems were: Km1 = 1.5 microM, V1 = 4.0 nmol x (g wet weight)-1 x (20 min)-1 (high-affinity uptake): Km2 = 800 microM, V2 = 260 nmol x (g wet weight)-1 x (20 min)-1 (low-affinity uptake). 4. Uptake mediated by each of the carrier systems was concentrative, entirely Na+-dependent, and required activation energies characteristic for active transport. 5. High-affinity transport was structurally specific for GABA. The substrate specificity of low-affinity uptake resembled that of beta-amino acid transport systems.

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References

Holzer P, HAGMULLER K . Transient apnoea after systemic injection of GABA in the rat. Naunyn Schmiedebergs Arch Pharmacol. 1979; 308(1):55-60. DOI: 10.1007/BF00499719. View

Cutler R, Young J . Bidirectional movement of gamma-aminobutyric acid in cerebral cortex slices incubated in small volumes of medium. Brain Res. 1979; 165(2):261-70. DOI: 10.1016/0006-8993(79)90558-4. View

East J, Dutton G, Currie D . Transport of GABA, beta-alanine and glutamate into perikarya of postnatal rat cerebellum. J Neurochem. 1980; 34(3):523-30. DOI: 10.1111/j.1471-4159.1980.tb11176.x. View

Martin D . Kinetics of the sodium-dependent transport of gamma-aminobutyric acid by synaptosomes. J Neurochem. 1973; 21(2):345-56. DOI: 10.1111/j.1471-4159.1973.tb04255.x. View

Iversen L, Kelly J . Uptake and metabolism of gamma-aminobutyric acid by neurones and glial cells. Biochem Pharmacol. 1975; 24(9):933-8. DOI: 10.1016/0006-2952(75)90422-0. View

Schrier B, Thompson E . On the role of glial cells in the mammalian nervous system. Uptake, excretion, and metabolism of putative neurotransmitters by cultured glial tumor cells. J Biol Chem. 1974; 249(6):1769-80. View

Taniguchi H, Okada Y, Seguchi H, Shimada C, Seki M, Tsutou A . High concentration of gamma-aminobutyric acid in pancreatic beta cells. Diabetes. 1979; 28(7):629-33. DOI: 10.2337/diab.28.7.629. View

Kulakowski E, Maturo J, Schaffer S . The identification of taurine receptors from rat heart sarcolemma. Biochem Biophys Res Commun. 1978; 80(4):936-41. DOI: 10.1016/0006-291x(78)91335-9. View

Larsson O, Krogsgaard-Larsen P, Schousboe A . High-affinity uptake of (RS)-nipecotic acid in astrocytes cultured from mouse brain. Comparison with GABA transport. J Neurochem. 1980; 34(4):970-7. DOI: 10.1111/j.1471-4159.1980.tb09673.x. View

10.

Hertz L, Wu P, Schousboe A . Evidence for net uptake of GABA into mouse astrocytes in primary cultures--its sodium dependence and potassium independence. Neurochem Res. 1978; 3(3):313-23. DOI: 10.1007/BF00965577. View

11.

Roskoski Jr R . Net uptake of L-glutamate and GABA by high affinity synaptosomal transport systems. J Neurochem. 1978; 31(2):493-8. DOI: 10.1111/j.1471-4159.1978.tb02663.x. View

12.

Bohlen P, Huot S, Palfreyman M . The relationship between GABA concentrations in brain and cerebrospinal fluid. Brain Res. 1979; 167(2):297-305. DOI: 10.1016/0006-8993(79)90824-2. View

13.

Levi G, Raiteri M . Exchange of neurotransmitter amino acid at nerve endings can simulate high affinity uptake. Nature. 1974; 250(5469):735-7. DOI: 10.1038/250735a0. View

14.

HOFSTEE B . On the evaluation of the constants Vm and KM in enzyme reactions. Science. 1952; 116(3013):329-31. DOI: 10.1126/science.116.3013.329. View

15.

Roberts P . Gamma-aminobutyric acid homoexchange in sensory ganglia. Brain Res. 1976; 113(1):206-9. DOI: 10.1016/0006-8993(76)90021-4. View

16.

Blaustein M, King A . Influence of membrane potential on the sodium-dependent uptake of gamma-aminobutyric acid by presynaptic nerve terminals: experimental observations and theoretical considerations. J Membr Biol. 1976; 30(2):153-73. DOI: 10.1007/BF01869665. View

17.

Wheeler D, Hollingsworth R . A model of GABA transport by cortical synaptosomes from the Long-Evans rat. J Neurosci Res. 1979; 4(4):265-89. DOI: 10.1002/jnr.490040405. View

18.

Defeudis F, Ossola L, Elkouby A, Wolff P, Mandel P . Effects of beta-alanine, L-2,4-diaminobutyric acid and nipecotic acid on the sodium-dependent binding of [3H]GABA to brain particles. Gen Pharmacol. 1979; 10(6):423-6. DOI: 10.1016/0306-3623(79)90001-6. View

19.

Hammerman M, SACKTOR B . Transport of beta-alanine in renal brush border membrane vesicles. Biochim Biophys Acta. 1978; 509(2):338-47. DOI: 10.1016/0005-2736(78)90052-4. View

20.

Kletzien R, Perdue J . Sugar transport in chick embryo fibroblasts. I. A functional change in the plasma membrane associated with the rate of cell growth. J Biol Chem. 1974; 249(11):3366-74. View