Detection of Staphylococcal Enterotoxins A, B, C, D, and E in Foods by Radioimnunoassay, Using Staphyloccal Cells Containing Protein A As Immunoadsorbent

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1978 Sep 1

PMID 727775

Citations 15

Authors

B A Miller

R F Reiser

M S Bergdoll

Affiliations

Soon will be listed here.

Abstract

A sensitive radioimmunoassay utilizing Staphylococcus aureus cells containing protein A as a coprecipitant was developed for the detection and quantitation of staphylococcal enterotoxins A, B, C, D, and E in a variety of foods. The enterotoxins were extracted from the foods by a simple and rapid procedure. The sensitivity of the assay is 1.0 ng or less of enterotoxin per g of food.

Citing Articles

16 Methods for Detecting Microbial Pathogens in Food and Water.

Kaspar C, Tartera C Methods Microbiol. 2024; 22:497-531.

PMID: 38620159 PMC: 7148849. DOI: 10.1016/S0580-9517(08)70253-8.

Staphylococcal enterotoxin production in the presence of non-enterotoxigenic staphylococci.

Noleto A, Bergdoll M Appl Environ Microbiol. 1980; 39(6):1167-71.

PMID: 7406485 PMC: 291501. DOI: 10.1128/aem.39.6.1167-1171.1980.

Determination of staphylococcal enterotoxin A in cheddar cheese produced without starter activity.

Ibrahim G, RADFORD H, Fell L Appl Environ Microbiol. 1980; 39(6):1134-7.

PMID: 7406483 PMC: 291495. DOI: 10.1128/aem.39.6.1134-1137.1980.

Enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in foods.

Freed R, Evenson M, Reiser R, Bergdoll M Appl Environ Microbiol. 1982; 44(6):1349-55.

PMID: 7159082 PMC: 242195. DOI: 10.1128/aem.44.6.1349-1355.1982.

Direct skin test in highly sensitized guinea pigs for rapid and sensitive determination of staphylococcal enterotoxin B.

Scheuber P, Mossmann H, Beck G, Hammer D Appl Environ Microbiol. 1983; 46(6):1351-6.

PMID: 6660875 PMC: 239575. DOI: 10.1128/aem.46.6.1351-1356.1983.

References

Orth D . Iodination of staphylococcal enterotoxin B by use of chloramine-T. Appl Environ Microbiol. 1977; 33(4):824-8. PMC: 170774. DOI: 10.1128/aem.33.4.824-828.1977. View

Orth D . Statistical analysis and quality control in radioimmunoassays for staphylococcal enterotoxins A, B, and C. Appl Environ Microbiol. 1977; 34(6):710-4. PMC: 242735. DOI: 10.1128/aem.34.6.710-714.1977. View

Niskanen A, Lindroth S . Preparation of labeled staphylococcal enterotoxin A with high specific activity. Appl Environ Microbiol. 1976; 32(6):735-40. PMC: 170453. DOI: 10.1128/aem.32.6.735-740.1976. View

Robern H, Dighton M, Yano Y, Dickie N . Double-antibody radioimmunoassay for staphylococcal enterotoxin C2. Appl Microbiol. 1975; 30(4):525-9. PMC: 187224. DOI: 10.1128/am.30.4.525-529.1975. View

Kessler S . Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975; 115(6):1617-24. View

KAUFFMAN P, Johnson H . Stability of 125I-labeled staphylococcal enterotoxins in solid-phase radioimmunoassay. Appl Microbiol. 1975; 29(6):776-9. PMC: 187078. DOI: 10.1128/am.29.6.776-779.1975. View

Bukovic J, Johnson H . Staphylococcal enterotoxin C: solid-phase radioimmunoassay. Appl Microbiol. 1975; 30(4):700-1. PMC: 187251. DOI: 10.1128/am.30.4.700-701.1975. View

Hunter R . Standardization of the chloramine-T method of protein iodination. Proc Soc Exp Biol Med. 1970; 133(3):989-92. DOI: 10.3181/00379727-133-34611. View

Collins 2nd W, METZGER J, Johnson A . A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin. J Immunol. 1972; 108(3):852-6. View

10.

COLLINS W, Johnson A, METZGER J, Bennett R . Rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A. Appl Microbiol. 1973; 25(5):774-7. PMC: 380910. DOI: 10.1128/am.25.5.774-777.1973. View

11.

Johnson H, Bukovic J, Kauffmann P . Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food. Appl Microbiol. 1973; 26(3):309-13. PMC: 379780. DOI: 10.1128/am.26.3.309-313.1973. View

12.

REISER R, Conaway D, Bergdoll M . Detection of staphylococcal enterotoxin in foods. Appl Microbiol. 1974; 27(1):83-5. PMC: 379972. DOI: 10.1128/am.27.1.83-85.1974. View

13.

Gruber J, Wright G . Ammonium sulfate coprecipitation antibody determination with purified staphylococcal enterotoxins. J Bacteriol. 1969; 99(1):18-24. PMC: 249961. DOI: 10.1128/jb.99.1.18-24.1969. View

14.

Johnson H, Bukovic J, KAUFFMAN P, Peeler J . Staphylococcal enterotoxin B: solid-phase radioimmunoassay. Appl Microbiol. 1971; 22(5):837-41. PMC: 376429. DOI: 10.1128/am.22.5.837-841.1971. View

15.

Thorell J, Johansson B . Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim Biophys Acta. 1971; 251(3):363-9. DOI: 10.1016/0005-2795(71)90123-1. View

16.

Berson S, Yalow R, Bauman A, Rothschild M, NEWERLY K . Insulin-I131 metabolism in human subjects: demonstration of insulin binding globulin in the circulation of insulin treated subjects. J Clin Invest. 1956; 35(2):170-90. PMC: 438794. DOI: 10.1172/JCI103262. View

17.

GREENWOOD F, Hunter W, GLOVER J . THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963; 89:114-23. PMC: 1202279. DOI: 10.1042/bj0890114. View

18.

CASMAN E, Bennett R . DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD. Appl Microbiol. 1965; 13:181-9. PMC: 1058218. DOI: 10.1128/am.13.2.181-189.1965. View

19.

Pober Z, Silverman G . Modified radioimmunoassay determination for staphylococcal enterotoxin B in foods. Appl Environ Microbiol. 1977; 33(3):620-5. PMC: 170734. DOI: 10.1128/aem.33.3.620-625.1977. View