The Energy Dispersive X-ray Microanalysis Technique in the Study of Fluids and Tissues of the Brain and the Inner Ear
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Abstract
The temporal bone from the fetal and adult mouse and brain from fetal and adult rat were cryosectioned in a conventional cryostat and -30 degrees C using 16 micrometers thick sections. The elemental composition was analyzed in the fluid-filled spaces of the membranous labyrinth and ventricular cavities of the brain. After drying these spaces containing small crystal-like aggregations of material which morphologically differed depending on their elemental composition. This changed during embryonic maturation. The qualitative X-ray analytical microscopy seems to be a reliable method to follow changes in the elemental composition of fluids.
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