» Articles » PMID: 7275691

The Energy Dispersive X-ray Microanalysis Technique in the Study of Fluids and Tissues of the Brain and the Inner Ear

Overview
Journal Histochemistry
Specialty Biochemistry
Date 1981 Jan 1
PMID 7275691
Authors
Affiliations
Soon will be listed here.
Abstract

The temporal bone from the fetal and adult mouse and brain from fetal and adult rat were cryosectioned in a conventional cryostat and -30 degrees C using 16 micrometers thick sections. The elemental composition was analyzed in the fluid-filled spaces of the membranous labyrinth and ventricular cavities of the brain. After drying these spaces containing small crystal-like aggregations of material which morphologically differed depending on their elemental composition. This changed during embryonic maturation. The qualitative X-ray analytical microscopy seems to be a reliable method to follow changes in the elemental composition of fluids.

References
1.
WROBLEWSKI R, Roomans G, Jansson E, Edstrom L . Electron probe X-ray microanalysis of human muscle biopsies. Histochemistry. 1978; 55(4):281-92. DOI: 10.1007/BF00508796. View

2.
Dempsey G, Bullivant S . A copper block method for freezing non-cryoprotected tissue to produce ice-crystal-free regions for electron microscopy. I. Evaluation using freeze-substitution. J Microsc. 1976; 106(3):251-60. DOI: 10.1111/j.1365-2818.1976.tb02405.x. View

3.
Anniko M, WROBLEWSKI R . Elemental composition of the developing inner ear. Ann Otol Rhinol Laryngol. 1981; 90(1 Pt 1):25-32. DOI: 10.1177/000348948109000107. View

4.
Barnard T, Seveus L . Preparation of biological material for X-ray microanalysis of diffusible elements. II. Comparison of different methods of drying ultrathin cryosections cut without a trough liquid. J Microsc. 1978; 112(3):281-91. DOI: 10.1111/j.1365-2818.1978.tb00078.x. View

5.
Saubermann A . Application of cryosectioning to x-ray microanalysis of biological tissue. Scan Electron Microsc. 1980; (Pt 2):421-30, 420. View