[Long-term Cultures in Polycythaemia Vera As Proof for Dependence on Erythropoietin (author's Transl)]

Overview

Journal Blut

Specialty Hematology

Date 1981 Jul 1

PMID 7260404

Citations 1

Authors

E Heilmann

A Holzknecht

H Fahrenkrug

U ESSERS

Affiliations

Soon will be listed here.

Abstract

In eight patients suffering from polycythaemia vera, the proliferation of erythroid colonies in methylcellulose with and without exogenous erythropoietin was studied. After three and five weeks, respectively, no colonies grew. Due to addition of erythropoietin more erythroid colonies proliferated which could be cultivated over nine weeks. The experiments suggest that the erythropoiesis in polycythaemia vera depends on erythropoietin.

Citing Articles

Burst-stimulating activity in sera of patients with polycythemia vera.

ESSERS U, Niedeggen A, Heilmann E, Hovels-Gurich H Blut. 1982; 45(2):103-8.

PMID: 7104495 DOI: 10.1007/BF00319937.

References

Nissen C, Cornu P, Weber W, Speck B . [Differential diagnosis of primary and secondary erythrocytosis by means of in vitro culture of hematopoietic stem cells]. Schweiz Med Wochenschr. 1978; 108(41):1581-3. View

Prchal J, AXELRAD A . Letter: Bone-marrow responses in polycythemia vera. N Engl J Med. 1974; 290(24):1382. DOI: 10.1056/nejm197406132902419. View

Guilbert L, Iscove N . Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoietic cell cultures. Nature. 1976; 263(5578):594-5. DOI: 10.1038/263594a0. View

Adamson J, Finch C . Erythropoietin and the polycythemias. Ann N Y Acad Sci. 1968; 149(1):560-3. DOI: 10.1111/j.1749-6632.1968.tb15195.x. View

Adamson J . The erythropoietin-hematocrit relationship in normal and polycythemic man: implications of marrow regulation. Blood. 1968; 32(4):597-609. View

Adamson J, Fialkow P, Murphy S, Prchal J, Steinmann L . Polycythemia vera: stem-cell and probable clonal origin of the disease. N Engl J Med. 1976; 295(17):913-6. DOI: 10.1056/NEJM197610212951702. View

KLUGE N, Knebel A, Beckmann H . [Polycythemia vera: neoplastic transformation of bone marrow cells in vitro]. Verh Dtsch Ges Inn Med. 1973; 79:441-4. View

Clarke B, Housman D . Characterization of an erythroid precursor cell of high proliferative capacity in normal human peripheral blood. Proc Natl Acad Sci U S A. 1977; 74(3):1105-9. PMC: 430608. DOI: 10.1073/pnas.74.3.1105. View

Lutton J, Levere R . Endogenous erythroid colony formation by peripheral blood mononuclear cells from patients with myelofibrosis and polycythemia vera. Acta Haematol. 1979; 62(2):94-9. DOI: 10.1159/000207547. View

10.

Zanjani E, Lutton J, Hoffman R, WASSERMAN L . Erythroid colony formation by polycythemia vera bone marrow in vitro. Dependence on erythropoietin. J Clin Invest. 1977; 59(5):841-8. PMC: 372292. DOI: 10.1172/JCI108706. View

11.

BERLIN N . Diagnosis and classification of the polycythemias. Semin Hematol. 1975; 12(4):339-51. View

12.

Iscove N, Senn J, Till J, McCulloch E . Colony formation by normal and leukemic human marrow cells in culture: effect of conditioned medium from human leukocytes. Blood. 1971; 37(1):1-5. View

13.

WARD H, Vautrin R, Kurnick J, Robinson W . Presence of a myeloproliferative factor in patients with polycythemia vera and agnogenic myeloid metaplasia. I. Expansion of the erythropoietin-responsive stem cell compartment. Proc Soc Exp Biol Med. 1974; 147(1):305-8. DOI: 10.3181/00379727-147-38331. View

14.

Prchal J, Adamson J, Murphy S, Steinmann L, Fialkow P . Polycythemia vera. The in vitro response of normal and abnormal stem cell lines to erythropoietin. J Clin Invest. 1978; 61(4):1044-7. PMC: 372621. DOI: 10.1172/JCI109003. View