Nuclear Ribonucleoprotein Particles from Adenovirus Infected Hela Cells

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 1981 May 22

PMID 7254204

Citations 1

Authors

J M Blanchard

J Weber

Affiliations

Soon will be listed here.

Abstract

Heterogenous nuclear RNA-protein complexes (hnRNP) from adenovirus-2 (Ad-2) infected Hela cells contain most of the virus-specific RNA which is labeled in the nucleus during periods lasting from 45 seconds to 2 hours. Moreover, the percentage of RNA which is Ad-2 specific as monitored by filter hybridization increases progressively from early to late period where it accounts for as much as 50-60% of the labeled RNA. The Ad-2 sequences are found in heterogenous complexes sedimenting between 30 and 200 S the density of which in CsCl (rho approximately 1.39) as well as metrizamide (rho approximately 1.29) seems to be the same as that of the bulk particles. A more detailed analysis with restriction fragments shows that all regions of the Ad-2 genome are represented in these particles.

Citing Articles

The orderly splicing of the first three leaders of the adenovirus-2 major late transcript.

Keohavong P, Gattoni R, LeMoullec J, Jacob M, Stevenin J Nucleic Acids Res. 1982; 10(4):1215-29.

PMID: 6175954 PMC: 320520. DOI: 10.1093/nar/10.4.1215.

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