Electrochemical Titrations of a Ferredoxin-ferredoxin:NADP+ Oxidoreductase Complex

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 1981 Apr 13

PMID 7236672

Citations 4

Authors

J M Smith

W H Smith

D B Knaff

Affiliations

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Abstract

Potentiometric titrations employing an electrochemical thin-layer cell indicate that complex formation between ferredoxin and ferredoxin:NADP+ oxidoreductase alters the midpoint oxidation-reduction potentials of both proteins. The midpoint potential of ferredoxin the complex becomes 22 +/- 6 mV more negative compared to ferredoxin alone while the midpoint potential of ferredoxin:NADP+ oxidoreductase becomes 23 +/- 4 mV more positive on complex formation.

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