Binding of Microtubule Protein to DNA and Chromatin: Possibility of Simultaneous Linkage of Microtubule to Nucleic and Assembly of the Microtubule Structure

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1981 Feb 25

PMID 7232207

Citations 13

Authors

A Villasante

V G Corces

J Avila

Affiliations

Soon will be listed here.

Abstract

Microtubule protein binds to DNA through microtubule associated polypeptides (MAPs). Among MAPs there is one high molecular weight polypeptide (MAP2) which interacts with DNA fundamentally through certain polynucleotide sequences. This interaction is not affected by the presence of histones and other chromosomal proteins. DNA can associate to assembled microtubules and when a determinate DNA/protein ratio is reached the nucleic acid behaves as a microtubule associated molecule. The nucleic acid fragments which preferentially bind to microtubules have been isolated and characterized. These fragments contain DNA regions enriched in repetitive sequences that hybridizes preferentially to the pericentromeric zone of metaphase chromosomes. These results give further support to the model of interaction microtubule-chromosome based upon the mediator function of the microtubule associated proteins.

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References

Jones O, Berg P . Studies on the binding of RNA polymerase to polynucleotides. J Mol Biol. 1966; 22(2):199-209. DOI: 10.1016/0022-2836(66)90126-4. View

Todaro G, Green H . Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963; 17:299-313. PMC: 2106200. DOI: 10.1083/jcb.17.2.299. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Shelanski M, Gaskin F, Cantor C . Microtubule assembly in the absence of added nucleotides. Proc Natl Acad Sci U S A. 1973; 70(3):765-8. PMC: 433354. DOI: 10.1073/pnas.70.3.765. View

Botchan M, McKenna G, Sharp P . Cleavage of mouse DNA by a restriction enzyme as a clue to the arrangement of genes. Cold Spring Harb Symp Quant Biol. 1974; 38:383-95. DOI: 10.1101/sqb.1974.038.01.041. View

Alfageme C, Zweidler A, Mahowald A, Cohen L . Histones of Drosophila embryos. Electrophoretic isolation and structural studies. J Biol Chem. 1974; 249(12):3729-36. View

Lacy E, Axel R . Analysis of DNA of isolated chromatin subunits. Proc Natl Acad Sci U S A. 1975; 72(10):3978-82. PMC: 433121. DOI: 10.1073/pnas.72.10.3978. View

Telzer B, MOSES M, Rosenbaum J . Assembly of microtubules onto kinetochores of isolated mitotic chromosomes of HeLa cells. Proc Natl Acad Sci U S A. 1975; 72(10):4023-7. PMC: 433130. DOI: 10.1073/pnas.72.10.4023. View

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

10.

Murphy D, Vallee R, Borisy G . Identity and polymerization-stimulatory activity of the nontubulin proteins associated with microtubules. Biochemistry. 1977; 16(12):2598-605. DOI: 10.1021/bi00631a004. View

11.

Corces V, Salas J, Salas M, Avila J . Binding of microtubule proteins to DNA: specificity of the interaction. Eur J Biochem. 1978; 86(2):473-9. DOI: 10.1111/j.1432-1033.1978.tb12330.x. View

12.

Wiche G, Corces V, Avila J . Preferential binding of hog brain microtubule-associated proteins to mouse satellite versus bulk DNA preparations. Nature. 1978; 273(5661):403-5. DOI: 10.1038/273403a0. View

13.

VATER W, Muller H, Unger E . Inhibition of microtubule formation by DNA. Biochem Biophys Res Commun. 1978; 84(3):721-6. DOI: 10.1016/0006-291x(78)90764-7. View

14.

Herzog W, Weber K . Fractionation of brain microtubule-associated proteins. Isolation of two different proteins which stimulate tubulin polymerization in vitro. Eur J Biochem. 1978; 92(1):1-8. DOI: 10.1111/j.1432-1033.1978.tb12716.x. View

15.

Corces V, Manso R, de la Torre J, Avila J, Nasr A, Wiche G . Effects of DNA on microtubule assembly. Eur J Biochem. 1980; 105(1):7-16. DOI: 10.1111/j.1432-1033.1980.tb04468.x. View

16.

Villasante A, Avila J . Incorporation of the high-molecular-weight microtubule-associated protein 2 (MAP2) into microtubules at steady state in vitro. Eur J Biochem. 1980; 105(2):307-13. DOI: 10.1111/j.1432-1033.1980.tb04502.x. View

17.

Gould R, Borisy G . Quantitative initiation of microtubule assembly by chromosomes from Chinese hamster ovary cells. Exp Cell Res. 1978; 113(2):369-74. DOI: 10.1016/0014-4827(78)90377-4. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

19.

Jones K . Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature. 1970; 225(5236):912-5. DOI: 10.1038/225912a0. View