Effects of Temperature on the Transport of Galactose in Human Erythrocytes

Overview

Journal J Physiol

Specialty Physiology

Date 1978 Sep 1

PMID 722542

Citations 5

Authors

H Ginsburg

S Yeroushalmy

Affiliations

Soon will be listed here.

Abstract

The transport of galactose in human erythrocytes has been resolved recently into a mechanism which involves two asymmetric carriers operating in antiparallel fashion. The effects of temperature on this mediated transport system in the range of 0--25 degrees C show the following features. The Michaelis constants for zero-trans influx and efflux and for equilibrium-exchange efflux, do not vary with temperature. Arrhenius plots of the maximal velocities show breaks between 3 and 15 degrees C with activation energies two- to threefold larger below the break than above it. The relative contribution of the two types of carriers to the total transport rate is not affected by temperature. The kinetic properties of the prevalent type of carriers are analysed in terms of the simple carrier model as formulated by Lieb & Stein (1974). This analysis shows that the relative concentration of the unloaded carrier at the inner interface of the membrane increases upon cooling. The free energy of translocation of the unloaded carrier and the change in entropy involved in this step are significantly larger in the low temperature range (0--5 degrees C) than in the higher range (15--25 degrees C). The results are discussed briefly in terms of possible lipid-protein interaction and the physicochemical nature of the erythrocyte membrane.

Citing Articles

Transmembrane Exchange of Fluorosugars: Characterization of Red Cell GLUT1 Kinetics Using F NMR.

Shishmarev D, Fontenelle C, Kuprov I, Linclau B, Kuchel P Biophys J. 2018; 115(10):1906-1919.

PMID: 30366625 PMC: 6303418. DOI: 10.1016/j.bpj.2018.09.030.

Kinetics of glucose transport in human erythrocytes.

Brahm J J Physiol. 1983; 339:339-54.

PMID: 6887027 PMC: 1199165. DOI: 10.1113/jphysiol.1983.sp014720.

Monocarboxylate transport in erythrocytes.

DEUTICKE B J Membr Biol. 1982; 70(2):89-103.

PMID: 6764785 DOI: 10.1007/BF01870219.

Glucose transport in a kinaseless Saccharomyces cerevisiae mutant.

Lang J, CIRILLO V J Bacteriol. 1987; 169(7):2932-7.

PMID: 3298207 PMC: 212329. DOI: 10.1128/jb.169.7.2932-2937.1987.

Temperature sensitivity of the extraneuronal uptake and metabolism of isoprenaline in the perfused rate heart.

Bonisch H, Uhlig W, Trendelenburg U Naunyn Schmiedebergs Arch Pharmacol. 1979; 306(3):229-39.

PMID: 471076 DOI: 10.1007/BF00507108.

References

DAWSON A, WIDDAS W . VARIATIONS WITH TEMPERATURE AND PH OF THE PARAMETERS OF GLUCOSE TRANSFER ACROSS THE ERYTHROCYTE MEMBRANE IN THE FOETAL GUINEA-PIG. J Physiol. 1964; 172:107-22. PMC: 1368906. DOI: 10.1113/jphysiol.1964.sp007406. View

MAWE R, HEMPLING H . The exchange of C14 glucose across the membrane of the human erythrocyte. J Cell Physiol. 1965; 66(1):95-103. DOI: 10.1002/jcp.1030660110. View

Thilo L, Trauble H, Overath P . Mechanistic interpretation of the influence of lipid phase transitions on transport functions. Biochemistry. 1977; 16(7):1283-90. DOI: 10.1021/bi00626a007. View

Kalish D, COHEN C, Jacobson B, Branton D . Membrane isolation on polylysine-coated glass beads. Asymmetry of bound membrane. Biochim Biophys Acta. 1978; 506(1):97-110. DOI: 10.1016/0005-2736(78)90437-6. View

Cabantchik Z, Ginsburg H . Transport of uridine in human red blood cells. Demonstration of a simple carrier-mediated process. J Gen Physiol. 1977; 69(1):75-96. PMC: 2215042. DOI: 10.1085/jgp.69.1.75. View

Aloni B, Shinitzky M, Livne A . Dynamics of erythrocyte lipids in intact cells, in ghost membranes and in liposomes. Biochim Biophys Acta. 1974; 348(3):438-41. DOI: 10.1016/0005-2760(74)90223-9. View

Jung C, Carlson L, Whaley D . Glucose transport carrier activities in extensively washed human red cell ghosts. Biochim Biophys Acta. 1971; 241(2):613-27. DOI: 10.1016/0005-2736(71)90059-9. View

BRETSCHER M . Asymmetrical lipid bilayer structure for biological membranes. Nat New Biol. 1972; 236(61):11-2. DOI: 10.1038/newbio236011a0. View

Jung C . Inactivation of glucose carriers in human erythrocyte membranes by 1-fluoro-2,4-dinitrobenzene. J Biol Chem. 1974; 249(11):3568-73. View

10.

Ginsburg H, Stein W . Zero-trans and infinite-cis uptake of galactose in human erythrocytes. Biochim Biophys Acta. 1975; 382(3):353-68. DOI: 10.1016/0005-2736(75)90277-1. View

11.

ZWAAL R, Roelofsen B, Colley C . Localization of red cell membrane constituents. Biochim Biophys Acta. 1973; 300(2):159-82. DOI: 10.1016/0304-4157(73)90003-8. View

12.

Lieb W, Stein W . Carrier and non-carrier models for sugar transport in the human red blood cell. Biochim Biophys Acta. 1972; 265(2):187-207. DOI: 10.1016/0304-4157(72)90002-0. View

13.

Lieb W, Stein W . Testing and characterizing the simple carrier. Biochim Biophys Acta. 1974; 373(2):178-96. DOI: 10.1016/0005-2736(74)90144-8. View

14.

Gottlieb M, Eanes E . On phase transitions in erythrocyte membranes and extracted membrane lipids. Biochim Biophys Acta. 1974; 373(3):519-22. DOI: 10.1016/0005-2736(74)90033-9. View

15.

Lacko L, Wittke B, Geck P . The temperature dependence of the exchange transport of glucose in human erythrocytes. J Cell Physiol. 1973; 82(2):213-8. DOI: 10.1002/jcp.1040820209. View

16.

Taverna R, Langdon R . A new method for measuring glucose translocation through biological membranes and its application to human erythrocyte ghosts. Biochim Biophys Acta. 1973; 298(2):412-21. DOI: 10.1016/0005-2736(73)90368-4. View

17.

Edwards P . The inactivation by fluorodinitrobenzene of glucose transport across the human erythrocyte membrane. The effect of glucose inside or outside the cell. Biochim Biophys Acta. 1973; 307(2):415-8. DOI: 10.1016/0005-2736(73)90107-7. View

18.

Hankin B, Stein W . On the temperature dependence of initial velocities of glucose transport in the human red blood cell. Biochim Biophys Acta. 1972; 288(1):127-36. DOI: 10.1016/0005-2736(72)90230-1. View

19.

Hankin B, Lieb W, Stein W . Rejection criteria for the asymmetric carrier and their application to glucose transport in the human red blood cell. Biochim Biophys Acta. 1972; 288(1):114-26. DOI: 10.1016/0005-2736(72)90229-5. View

20.

Cabantchik Z, Rothstein A . The nature of the membrane sites controlling anion permeability of human red blood cells as determined by studies with disulfonic stilbene derivatives. J Membr Biol. 1972; 10(3):311-30. DOI: 10.1007/BF01867863. View