Effect of Fat and Microflora on Hepatic, Small Intestinal and Colonic HMG CoA Reductase, Cytochrome P450 and Cytochrome B5

Overview

Journal Lipids

Specialty Biochemistry

Date 1981 Mar 1

PMID 7219088

Citations 1

Authors

D Hanson

B S Reddy

Affiliations

Soon will be listed here.

Abstract

High levels of dietary fat caused a significant reduction in HMG CoA reductase activity in the liver of germ-free rats whereas significantly elevated small intestinal enzyme activity was observed. Dietary fat had no significant effect on HMG CoA reductase activity in any tissue studied in the conventional rat. No significant change in colonic HMG CoA reductase activity was observed between any of the experimental groups. Rats fed a high-fat diet tended to exhibit higher cytochrome P450 levels in all tissues studied, regardless of the presence of intestinal microflora.

Citing Articles

Microsomal ethanol oxidation in the colonic mucosa of the rat. Effect of chronic ethanol ingestion.

Seitz H, Bosche J, Czygan P, Veith S, KOMMERELL B Naunyn Schmiedebergs Arch Pharmacol. 1982; 320(1):81-4.

PMID: 6811958 DOI: 10.1007/BF00499078.

References

Strobel H, Fang W, Oshinsky R . Role of colonic cytochrome P-450 in large bowel carcinogenesis. Cancer. 1980; 45(5 Suppl):1060-5. DOI: 10.1002/1097-0142(19800315)45:5+<1060::aid-cncr2820451305>3.0.co;2-e. View

Asano T, Pollard M, Madsen D . Effects of cholestyramine on 1,2-dimethylhydrazine-induced enteric carcinoma in germfree rats. Proc Soc Exp Biol Med. 1975; 150(3):780-5. DOI: 10.3181/00379727-150-39124. View

WATTENBERG L . Carcinogen-detoxifying mechanisms in the gastrointestinal tract. Gastroenterology. 1966; 51(5):932-5. View

LAKSHMANAN M, Dugan R, NEPOKROEFF C, Ness G, Porter J . Regulation of rat liver beta-hydroxy-beta-methylglutaryl coenzyme. A reductase activity and cholesterol levels of serum and liver in various dietary and hormonal states. Arch Biochem Biophys. 1975; 168(1):89-95. DOI: 10.1016/0003-9861(75)90231-3. View

Bochenek W, Rodgers J . Dietary regulation of 3-hydroxy-3-methylglutaryl-CoA reductase from rat intestine. Biochim Biophys Acta. 1979; 575(1):57-62. DOI: 10.1016/0005-2760(79)90130-9. View

Jeske D, Dietschy J . Regulation of rates of cholesterol synthesis in vivo in the liver and carcass of the rat measured using [3H]water. J Lipid Res. 1980; 21(3):364-76. View

Dietschy J, SIPERSTEIN M . Effect of cholesterol feeding and fasting on sterol synthesis in seventeen tissues of the rat. J Lipid Res. 1967; 8(2):97-104. View

Evrard E, HOET P, EYSSEN H, Charlier H, SACQUET E . FAECAL LIPIDS IN GERM-FREE AND CONVENTIONAL RATS. Br J Exp Pathol. 1964; 45:409-14. PMC: 2094335. View

WOSTMANN B, WIECH N, Kung E . Catabolism and elimination of cholesterol in germfree rats. J Lipid Res. 1966; 7(1):77-82. View

10.

HEIDELBERGER C . Chemical carcinogenesis. Annu Rev Biochem. 1975; 44:79-121. DOI: 10.1146/annurev.bi.44.070175.000455. View

11.

Abrams G, Bauer H, SPRINZ H . Influence of the normal flora on mucosal morphology and cellular renewal in the ileum. A comparison of germ-free and conventional mice. Lab Invest. 1963; 12:355-64. View

12.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

13.

WATTENBERG L . Effects of dietary constituents on the metabolism of chemical carcinogens. Cancer Res. 1975; 35(11 Pt. 2):3326-31. View

14.

Wynder E, Shigematsu T . Environmental factors of cancer of the colon and rectum. Cancer. 1967; 20(9):1520-61. DOI: 10.1002/1097-0142(196709)20:9<1520::aid-cncr2820200920>3.0.co;2-3. View

15.

Reddy B, Wynder E . Metabolic epidemiology of colon cancer. Fecal bile acids and neutral sterols in colon cancer patients and patients with adenomatous polyps. Cancer. 1977; 39(6):2533-9. DOI: 10.1002/1097-0142(197706)39:6<2533::aid-cncr2820390634>3.0.co;2-x. View

16.

Sugano M, Ide T, Okamatsu H, Fujisaki Y, Takahara H . Dietary modification of the distribution of 3-hydroxy-3-methylglutaryl coenzyme A reductase and sterol synthesizing activities in the rat small intestine. J Nutr. 1980; 110(2):360-70. DOI: 10.1093/jn/110.2.360. View

17.

Goldfarb S, Pitot H . Stimulatory effect of dietary lipid and cholestyramine on hepatic HMG CoA reductase. J Lipid Res. 1972; 13(6):797-801. View

18.

Omura T, Sato R . THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE. J Biol Chem. 1964; 239:2370-8. View

19.

Armstrong B, DOLL R . Environmental factors and cancer incidence and mortality in different countries, with special reference to dietary practices. Int J Cancer. 1975; 15(4):617-31. DOI: 10.1002/ijc.2910150411. View

20.

Reddy B, Martin C, Wynder E . Fecal bile acids and cholesterol metabolites of patients with ulcerative colitis, a high-risk group for development of colon cancer. Cancer Res. 1977; 37(6):1697-701. View