The Link Between Metabolism and Active Transport of Sodium in Human Red Cell Ghosts

Overview

Journal J Membr Biol

Date 1980 Dec 15

PMID 7205943

Citations 6

Authors

J F Hoffman

Affiliations

Soon will be listed here.

Abstract

Reconstituted human red blood cell ghosts have been used to assay various metabolic intermediates for their role in driving the Na:K pump. ATP was identified as the primary substrate of the pump. The main evidence was based on 1) the finding that the only requirement for activation of the pump was the presence of ATP, whether incorporated directly or generated by an ATP-yielding reaction; 2) the discriminating effects of various metabolic inhibitors; 3) the competition between the pump and hexokinase reaction for ATP; and 4) the difference in effects of adenosine and inosine in activating the pump in energy-depleted ghosts. ADP was found to affect the Na:K pump due to the presence of an adenylate kinase and perhaps because of an effect on the phosphoryl potential. The sidedness of action of the cardiotonic steroid, strophanthidin, was evaluated and found to inhibit the Na:K pump only from the outside of the membrane. Inhibition of the pump by strophanthidin was also found to spare ATP in reconstituted ghosts provided the nonspecific phosphatase activity was suppressed.

Citing Articles

Coherent Behavior and the Bound State of Water and K(+) Imply Another Model of Bioenergetics: Negative Entropy Instead of High-energy Bonds.

Jaeken L, Vasilievich Matveev V Open Biochem J. 2012; 6:139-59.

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Identification of cytoskeletal elements enclosing the ATP pools that fuel human red blood cell membrane cation pumps.

Chu H, Puchulu-Campanella E, Galan J, Tao W, Low P, Hoffman J Proc Natl Acad Sci U S A. 2012; 109(31):12794-9.

PMID: 22745158 PMC: 3412019. DOI: 10.1073/pnas.1209014109.

Does tissue ATP content limit active sodium transport across intestinal epithelia in vitro?.

Grasl M, Krivanek P, Turnheim K Pflugers Arch. 1982; 395(3):257-9.

PMID: 7155800 DOI: 10.1007/BF00584820.

Direct high-resolution nuclear magnetic resonance studies of cation transport in vivo, Na+ transport in yeast cells.

Balschi J, CIRILLO V, Springer Jr C Biophys J. 1982; 38(3):323-6.

PMID: 7049258 PMC: 1328876. DOI: 10.1016/S0006-3495(82)84566-9.

[3H]Ouabain binding and Na+, K+-ATPase in resealed human red cell ghosts.

Shoemaker D, Lauf P J Gen Physiol. 1983; 81(3):401-20.

PMID: 6302199 PMC: 2215578. DOI: 10.1085/jgp.81.3.401.

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