Article: Preparation of a highly purified allergen from Dirofilaria immitis. Reaginic antibody formation in mice

A Dirofilaria immitis protein allergen was purified; it had a molecular weight of 15,000-20,000 and a carbohydrate content of 2%. The allergenic activity of adult Dirofilaria extracts was assayed by passive cutaneous anaphylaxis (PCA) in rats using mouse sera obtained by immunization with various fractions. The mouse-Dirofilaria system was used to study the degree of purification of allergen. The purified Dirofilaria allergen appeared as one band after sodium dodecyl sulphate polyacrylamide gel (SDS-gel) electrophoresis and one precipitin arc by immunoelectrophoresis (IEP). It was inclined to aggregate in buffered solution. The results suggested that the allergen--reagin axis was a simple single antigen-antibody interaction. Immunological responses to the purified allergen were compared among four inbred strains, two hybrid strains and two outbred strains of mice. They produced relatively high titres of reaginic antibody but did not produce detectable indirect haemagglutinating test (IHA) antibody. Among the strains tested, BALB/c was a high responder and also contained to produce the reaginic antibody for longer than the other strains.

Citing Articles

Recombinant Dirofilaria immitis polyprotein that stimulates murine B cells to produce nonspecific polyclonal immunoglobulin E antibody.

Tezuka H, Imai S, Muto R, Furuhashi Y, Fujita K Infect Immun. 2002; 70(3):1235-44.

PMID: 11854206 PMC: 127741. DOI: 10.1128/IAI.70.3.1235-1244.2002.

Purified excretory-secretory component of filarial parasite enhances Fc epsilon RII/CD23 expression on human splenic B and T cells and IgE synthesis while potentiating T-helper type 2-related cytokine generation from T cells.

Yamaoka K, Kolb J, Miyasaka N, Inuo G, Fujita K Immunology. 1994; 81(4):507-12.

PMID: 8039805 PMC: 1422388.

Purification and characterization of excretory and secretory antigen of Toxocara canis larvae.

Sugane K, Oshima T Immunology. 1983; 50(1):113-20.

PMID: 6411609 PMC: 1454224.

References

1.

Desowitz R, SAAVE J, Sawada T . Studies on the immuno-epidemiology of parasitic infections in New Guinea. I. Population studies on the relationship of a skin test to microfilaraemia. Ann Trop Med Parasitol. 1966; 60(3):257-64. DOI: 10.1080/00034983.1966.11686414. View

2.

SADUN E, DUXBURY R, Gore R, Stechschulte D . Homologous passive cutaneous anaphylactic and fluorescent antibody reactions in rabbits infected with Dirofilaria uniformis. J Infect Dis. 1967; 117(4):317-26. DOI: 10.1093/infdis/117.4.317. View

3.

Sawada T, Sato S, Matsuyama S, Miyagi H, Shinzato J . Intradermal skin test with antigen FST (FSCD1) on individuals in endemic area. Jpn J Exp Med. 1968; 38(6):405-14. View

4.

Ito K, Sawada T, Sato S . Increased serum IgE level in individuals infected with Schistosoma japonicum, Wuchereria bancrofti or hook worm, and the changes by treatment in schistosomiasis. Jpn J Exp Med. 1972; 42(2):115-23. View

5.

Hussain R, Bradbury S, STREJAN G . Hypersensitivity to Ascaris antigens. 8. Characterization of a highly purified allergen. J Immunol. 1973; 111(1):260-8. View

6.

Ambler J, Doe J, Gemmell D, Roberts J, Orr T . Biological techniques for studying the allergenic components of nematodes. I. Detection of allergenic components in Ascaris suum extracts. J Immunol Methods. 1972; 1(4):317-28. DOI: 10.1016/0022-1759(72)90025-7. View

7.

Fujita K . Separation of Dirofilaria immitis allergen from the IgG-inducing antigens. Jpn J Med Sci Biol. 1975; 28(2):139-49. DOI: 10.7883/yoken1952.28.139. View

8.

Harris W . The allergens of Schistosoma mansoni. II. Further separation by sephadex G-200 and ion-exchange chromatography. Immunology. 1975; 29(5):835-44. PMC: 1446000. View

9.

Benjamin D, Soulsby E . The homocytotropic and hemagglutinating antibody responses to Brugia pahangi infection in the multimammate rat (Mastomys natalensis). Am J Trop Med Hyg. 1976; 25(2):266-72. DOI: 10.4269/ajtmh.1976.25.266. View

10.

Condeelis J . A sodium dodecyl sulfate micro-gel-electrophoresis technique suitable for routine laboratory analysis. Anal Biochem. 1977; 77(1):195-207. DOI: 10.1016/0003-2697(77)90305-0. View

11.

Fujita K, Ikeda T, Tsukidate S . Immunological and physicochemical properties of a highly purified allergen from Dirofilaria immitis. Int Arch Allergy Appl Immunol. 1979; 60(2):121-31. DOI: 10.1159/000232333. View

12.

Davis B . DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964; 121:404-27. DOI: 10.1111/j.1749-6632.1964.tb14213.x. View

Preparation of a Highly Purified Allergen from Dirofilaria Immitis. Reaginic Antibody Formation in Mice