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Conversion of Isopenicillin N into Penicillin N in Cell-free Extracts of Cephalosporium Acremonium

Overview
Journal Biochem J
Specialty Biochemistry
Date 1981 Feb 15
PMID 7197923
Citations 7
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Abstract

In a cell-free system prepared by osmotic lysis of protoplasts of Cephalosporium acremonium, isopenicillin N is converted into penicillin N. The epimerase activity of the system is labile.

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References
1.
Smith B, Warren S, NEWTON G, ABRAHAM E . Biosynthesis of penicillin N and cephalosporin C. Antibiotic production and other features of the metabolism of Cephalosporium sp. Biochem J. 1967; 103(3):877-90. PMC: 1270493. DOI: 10.1042/bj1030877. View

2.
Davies R, ABRAHAM E . Separation, purification and properties of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9. Biochem J. 1974; 143(1):115-27. PMC: 1168359. DOI: 10.1042/bj1430115. View

3.
Usher J, LODER B, ABRAHAM E . Synthesis of tritium-labelled isopenicillin N, penicillin N and 6-aminopenicillanic acid. Biochem J. 1975; 151(3):729-39. PMC: 1172422. DOI: 10.1042/bj1510729. View

4.
Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

5.
ABRAHAM E, NEWTON G . Purification and some properties of cephalosporin N, a new penicillin. Biochem J. 1954; 58(1):94-102. PMC: 1269848. DOI: 10.1042/bj0580094. View