Species-specific Monoclonal Antibodies in the Assignment of the Gene for Human Fibronectin to Chromosome 2

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1982 Jan 1

PMID 7188364

Citations 6

Authors

L Zardi

M Cianfriglia

E Balza

B Carnemolla

A Siri

C M Croce

Affiliations

Soon will be listed here.

Abstract

Using three different species-specific monoclonal antibodies we have studied, in human-mouse and human-hamster somatic cell hybrids, the correlation between the presence of different human chromosomes and the ability to release human fibronectin into the tissue culture medium. Presence of human fibronectin was determined by an affinity-radioimmunoassay. In addition, tissue culture media of the different hybrids were separated on SDS-polyacrylamide gels, the proteins were blotted onto a nitrocellulose sheet and human fibronectin visualized by an immunoenzymatic technique. Karyology and determination of isoenzyme markers of specific human chromosomes show that the ability to produce human fibronectin segregated with the presence of human chromosome 2.

Citing Articles

Use of the uteroglobin platform for the expression of a bivalent antibody against oncofetal fibronectin in Escherichia coli.

Ventura E, Riondato M, Sambuceti G, Salis A, Damonte G, Cordazzo C PLoS One. 2013; 8(12):e82878.

PMID: 24367567 PMC: 3868587. DOI: 10.1371/journal.pone.0082878.

Alternative splicing of the angiogenesis associated extra-domain B of fibronectin regulates the accessibility of the B-C loop of the type III repeat 8.

Ventura E, Sassi F, Parodi A, Balza E, Borsi L, Castellani P PLoS One. 2010; 5(2):e9145.

PMID: 20161770 PMC: 2818841. DOI: 10.1371/journal.pone.0009145.

Monoclonal antibodies in the analysis of fibronectin isoforms generated by alternative splicing of mRNA precursors in normal and transformed human cells.

Borsi L, Carnemolla B, Castellani P, Rosellini C, Vecchio D, Allemanni G J Cell Biol. 1987; 104(3):595-600.

PMID: 3643927 PMC: 2114534. DOI: 10.1083/jcb.104.3.595.

Transformed human cells release different fibronectin variants than do normal cells.

Castellani P, Siri A, Rosellini C, Infusini E, Borsi L, Zardi L J Cell Biol. 1986; 103(5):1671-7.

PMID: 3023390 PMC: 2114372. DOI: 10.1083/jcb.103.5.1671.

Isolation and subregional mapping of an arbitrary cloned probe detecting a common RFLP on human chromosome 2.

Davatelis G Am J Hum Genet. 1985; 37(5):1015-21.

PMID: 2996333 PMC: 1684695.

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