A New Solid-phase Immunoradiometric Assay for Anti-thyroglobulin Autoantibody

Overview

Journal J Endocrinol Invest

Publisher Springer

Specialty Endocrinology

Date 1982 Jul 1

PMID 7175106

Citations 14

Authors

S Mariotti

S Pisani

A Russova

A Pinchera

Affiliations

Soon will be listed here.

Abstract

A newly developed sensitive and quantitative immunoradiometric assay (IRMA) for anti-thyroglobulin (anti-Tg) autoantibody is described. Serum samples to be tested are added to wells of polyvinyl microtiter plates coated with human thyroglobulin. After removal of the unbound material, anti-Tg antibody is determined by adding purified 125I-anti-human immunoglobulin G antibody. Using 1.0 microliter of serum anti-Tg antibody was detected in 81.2% of patients with Hashimoto's thyroiditis or idiopathic myxedema (n = 32), in 46.4% of those with Graves' disease (n = 28), in 11.9% of subjects with other thyroid disorders (n = 42) and in 4.2% of normal controls (n = 71). Similar percentages of positive tests were observed by passive hemagglutination (PH) and a good correlation was found between the antibody levels determined by the two techniques. Using larger amounts of serum (100 microliters) detectable anti-Tg antibody by IRMA was found in the majority of patients with thyroid autoimmune disorders who had negative PH tests. Quantitative measurements of anti-Tg antibody by IRMA could be obtained by using purified anti-Tg antibody as standard reference. The minimum detectable amount of anti-Tg antibody was 0.5 ng. The present method is proposed as a simple and convenient technique for quantitative measurement of any antibody, using wells coated with the appropriate antigen.

Citing Articles

Clinical significance of thyroglobulin antibodies and thyroid peroxidase antibodies in Graves' disease: a cross-sectional study.

Katahira M, Tsunekawa T, Mizoguchi A, Yamaguchi M, Tsuru K, Takashima H Hormones (Athens). 2023; 22(2):253-261.

PMID: 36811756 DOI: 10.1007/s42000-023-00437-7.

The Presence of Serum TgAb Suggests Lower Risks for Glucose and Lipid Metabolic Disorders in Euthyroid General Population From a National Survey.

Zhang J, Gao Y, Li Y, Teng D, Xue Y, Yan L Front Endocrinol (Lausanne). 2020; 11:139.

PMID: 32256451 PMC: 7093715. DOI: 10.3389/fendo.2020.00139.

Demographic, clinical, laboratory, ultrasonographic, and cytological features of patients with Hashimoto's thyroiditis: results of a university hospital of 769 patients in Turkey.

Erdogan M, Erdem N, Cetinkalp S, Ozgen A, Saygili F, Yilmaz C Endocrine. 2009; 36(3):486-90.

PMID: 19856136 DOI: 10.1007/s12020-009-9258-z.

Graves' ophthalmopathy: state of the art and perspectives.

Bartalena L, Wiersinga W, Pinchera A J Endocrinol Invest. 2004; 27(3):295-301.

PMID: 15165007 DOI: 10.1007/BF03345280.

Role of thyroglobulin in the pathogenesis of Graves' ophthalmopathy: the hypothesis of Kriss revisited.

Marino M, Chiovato L, Lisi S, Altea M, Marcocci C, Pinchera A J Endocrinol Invest. 2004; 27(3):230-6.

PMID: 15164998 DOI: 10.1007/BF03345271.

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