Ultrastructural Observations on the Regeneration of Adrenocortical Autotransplants in the Rat Spleen

Overview

Journal J Anat

Specialty Anatomy & Histology

Date 1982 Sep 1

PMID 7174500

Citations 4

Authors

A S Belloni

P Vassanelli

C Robba

P Rebuffat

G Mazzocchi

G G Nussdorfer

Affiliations

Soon will be listed here.

Abstract

The regeneration of adrenocortical autotransplants in the rat spleen has been investigated by light and electron microscopy. Up to the seventh day after implantation, adrenal grafts showed large areas of necrosis and contained many degenerating (apoptotic) adrenocortical cells, some mesenchyme-like poorly differentiated elements, and occasional viable parenchymal cells. These last cells possessed mitochondria with scanty tubular cristae and few profiles of smooth endoplasmic reticulum. After 15 days of regeneration, adrenal grafts were reduced in volume, but contained only viable adrenocortical cells; after 30-36 days, autotransplants were noticeably enlarged and surrounded by an evident connective tissue capsule. Regeneration was closely associated with the morphological differentiation of adrenocortical cells, which one month after transplantation were found to assume all the typical features of adult rat zona fasciculata elements (i.e. mitochondria with vesicular cristae, abundant smooth endoplasmic reticulum, some lipid droplets and a well developed Golgi apparatus).

Citing Articles

Involvement of DHH and GLI1 in adrenocortical autograft regeneration in rats.

Takizawa N, Tanaka S, Oe S, Koike T, Yoshida T, Hirahara Y Sci Rep. 2018; 8(1):14542.

PMID: 30266964 PMC: 6162278. DOI: 10.1038/s41598-018-32870-9.

Effects of inhibiting neonatal methamphetamine-induced corticosterone release in rats by adrenal autotransplantation on later learning, memory, and plasma corticosterone levels.

Grace C, Schaefer T, Graham D, Skelton M, Williams M, Vorhees C Int J Dev Neurosci. 2010; 28(4):331-42.

PMID: 20184951 PMC: 2873091. DOI: 10.1016/j.ijdevneu.2010.02.005.

Neonatal methamphetamine-induced corticosterone release in rats is inhibited by adrenal autotransplantation without altering the effect of the drug on hippocampal serotonin.

Grace C, Schaefer T, Gudelsky G, Williams M, Vorhees C Neurotoxicol Teratol. 2010; 32(3):356-61.

PMID: 20153424 PMC: 2854321. DOI: 10.1016/j.ntt.2010.02.001.

Biochemical evidence of the functional recovery and regeneration of adrenal autotransplants in the rat spleen.

Allende G, Chavira R Endocrine. 2002; 16(3):173-9.

PMID: 11954660 DOI: 10.1385/endo:16:3:173.

References

TAMAOKI B . Steroidogenesis and cell structure. Biochemical pursuit of sites of steroid biosynthesis. J Steroid Biochem. 1973; 4(1):89-118. DOI: 10.1016/0022-4731(73)90084-8. View

Wyllie A, KERR J, Currie A . Cell death: the significance of apoptosis. Int Rev Cytol. 1980; 68:251-306. DOI: 10.1016/s0074-7696(08)62312-8. View

Karnovsky M . Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961; 11:729-32. PMC: 2225134. DOI: 10.1083/jcb.11.3.729. View

Fortier C, de Groot J . Adenohypophysial corticotrophin and plasma free corticosteroids during regeneration of the enucleated rat adrenal gland. Am J Physiol. 1959; 196(3):589-92. DOI: 10.1152/ajplegacy.1959.196.3.589. View

Nakayama I, Nickerson P, SKELTON F . An ultrastructural study of the adrenocorticotrophic hormone-secreting cell in the rat adenohypophysis during adrenal cortical regeneration. Lab Invest. 1969; 21(2):169-78. View

Dal Lago A, Lucke S . A method of fixing rat testis for light and electron microscopy. Stain Technol. 1973; 48(6):289-95. DOI: 10.3109/10520297309116644. View

Seki M, Sekiyama S, Miyahara H, Ichii S . Studies on regenerating adrenal cortex. 2. Autoradiographic and electron microscopic observations. Endocrinol Jpn. 1969; 16(3):361-77. DOI: 10.1507/endocrj1954.16.361. View

Moses H, Davis W, Rosenthal A, GARREN L . Adrenal cholesterol: localization by electron-microscope autoradiography. Science. 1969; 163(3872):1203-5. DOI: 10.1126/science.163.3872.1203. View

Nussdorfer G, Mazzocchi G, MENEGHELLI V . Cytophysiology of the adrenal zona fasciculata. Int Rev Cytol. 1978; 55:291-365. DOI: 10.1016/s0074-7696(08)61891-4. View

10.

Wyllie A, KERR J, Macaskill I, Currie A . Adrenocortical cell deletion: the role of ACTH. J Pathol. 1973; 111(2):85-94. DOI: 10.1002/path.1711110203. View

11.

Penney D, PATT D, DIXON Jr W . THE FINE STRUCTURE OF REGENERATING ADRENOCORTICAL AUTOTRANSPLANTS IN THE RAT. Anat Rec. 1963; 146:319-35. DOI: 10.1002/ar.1091460406. View

12.

Armato U, Nussdorfer G . Tissue culture of rat adult decapsulated adrenal glands. A methodological, ultrastructural and morphometric investigation. Z Zellforsch Mikrosk Anat. 1972; 135(2):245-73. DOI: 10.1007/BF00315129. View

13.

Wyllie A, KERR J, Currie A . Cell death in the normal neonatal rat adrenal cortex. J Pathol. 1973; 111(4):255-61. DOI: 10.1002/path.1711110406. View

14.

YAGO N, Seki M, Sekiyama S, Kobayashi S, Kurokawa H . Growth and differentiation of mitochondria in the regenerating rat adrenal cortex. J Cell Biol. 1972; 52(3):503-13. PMC: 2108655. DOI: 10.1083/jcb.52.3.503. View