Bacteriophage T7 Defective in the Gene 6 Exonuclease Promotes Site-specific Cleavages of T7 DNA in Vivo and in Vitro

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1982 Oct 1

PMID 7143567

Citations 3

Authors

D D Lee

P D Sadowski

Affiliations

Soon will be listed here.

Abstract

Site-specific cleavages of intracellular DNA were demonstrated in bacteriophage T7 6am-infected cells. The sites of the cleavages were located at 46.8 and 68.7% (1% of the T7 DNA length = 400 base pairs) from the left end of the T7 genome. These cleavages required the products of genes 3 (endonuclease), 4 (DNA primase), and 5 (DNA polymerase). However, the product of gene 6 (exonuclease) must be absent. Site-specific cleavage was also shown to occur in vitro in extracts of T7 6am-infected cells, although at a different site: 82.8% from the left end of the T7 genome.

Citing Articles

In vivo cleavage of cytosine-containing bacteriophage T4 DNA to genetically distinct, discretely sized fragments.

Carlson K, Wiberg J J Virol. 1983; 48(1):18-30.

PMID: 6887350 PMC: 255318. DOI: 10.1128/JVI.48.1.18-30.1983.

In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay.

Lee D, Sadowski P J Virol. 1983; 48(3):647-53.

PMID: 6313964 PMC: 255396. DOI: 10.1128/JVI.48.3.647-653.1983.

Site-specific cleavage of bacteriophage T4 DNA associated with the absence of gene 46 product function.

Albright L, Geiduschek E J Virol. 1983; 47(1):77-88.

PMID: 6306283 PMC: 255202. DOI: 10.1128/JVI.47.1.77-88.1983.

References

Studier F . The genetics and physiology of bacteriophage T7. Virology. 1969; 39(3):562-74. DOI: 10.1016/0042-6822(69)90104-4. View

CENTER M, Studier F, Richardson C . The structural gene for a T7 endonuclease essential for phage DNA synthesis. Proc Natl Acad Sci U S A. 1970; 65(1):242-8. PMC: 286216. DOI: 10.1073/pnas.65.1.242. View

CENTER M, Richardson C . An endonuclease induced after infection of Escherichia coli with bacteriophage T7. I. Purification and properties of the enzyme. J Biol Chem. 1970; 245(23):6285-91. View

Sadowski P . Bacteriophage T7 endonuclease. I. Properties of the enzyme purified from T7 phage-infected Escherichia coli B. J Biol Chem. 1971; 246(1):209-16. View

Sadowski P, Kerr C . Degradation of Escherichia coli B deoxyribonucleic acid after infection with deoxyribonucleic acid-defective amber mutants of bacteriophage T7. J Virol. 1970; 6(2):149-55. PMC: 376101. DOI: 10.1128/JVI.6.2.149-155.1970. View

Kerr C, Sadowski P . Gene 6 exonuclease of bacteriophage T7. I. Purification and properties of the enzyme. J Biol Chem. 1972; 247(1):305-10. View

Kerr C, Sadowski P . Gene 6 exonuclease of bacteriophage T7. II. Mechanism of the reaction. J Biol Chem. 1972; 247(1):311-8. View

Meselson M, Radding C . A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975; 72(1):358-61. PMC: 432304. DOI: 10.1073/pnas.72.1.358. View

Powling A, Knippers R . Some functions involved in bacteriophage T7 genetic recombination. Mol Gen Genet. 1974; 134(2):173-80. DOI: 10.1007/BF00268418. View

10.

Kerr C, Sadowski P . The involvement of genes 3,4,5 and 6 in genetic recombination in bacteriophage T7. Virology. 1975; 65(1):281-5. DOI: 10.1016/0042-6822(75)90031-8. View

11.

Sadowski P, Vetter D . Genetic recombination of bacteriophage T7 DNA in vitro. Proc Natl Acad Sci U S A. 1976; 73(3):692-6. PMC: 335984. DOI: 10.1073/pnas.73.3.692. View

12.

Miller Jr R, Lee M . The role of bacteriophage T7 exonuclease (gene 6) in genetic recombination and production of concatemers. J Mol Biol. 1976; 101(2):223-34. DOI: 10.1016/0022-2836(76)90374-0. View

13.

Powling A, Knippers R . Recombination of bacteriophage T7 in vivo. Mol Gen Genet. 1976; 149(1):63-71. DOI: 10.1007/BF00275961. View

14.

Tsujimoto Y, Ogawa H . EcoRI-sensitive mutation of T7 phage. Mol Gen Genet. 1977; 150(2):221-3. DOI: 10.1007/BF00695402. View

15.

Bingham A, Sharman A, Atkinson T . The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine. FEBS Lett. 1977; 76(2):250-6. DOI: 10.1016/0014-5793(77)80162-2. View

16.

Shinozaki K, Okazaki T . RNA-linked nascent DNA pieces in T7 phage-infected Escherichia coli cells. I. Role of gene 6 exonuclease in removal of the linked RNA. Mol Gen Genet. 1977; 154(3):263-7. DOI: 10.1007/BF00571281. View

17.

Sugino A, Peebles C, Kreuzer K, Cozzarelli N . Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme. Proc Natl Acad Sci U S A. 1977; 74(11):4767-71. PMC: 432036. DOI: 10.1073/pnas.74.11.4767. View

18.

Gellert M, Mizuuchi K, ODea M, Itoh T, TOMIZAWA J . Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity. Proc Natl Acad Sci U S A. 1977; 74(11):4772-6. PMC: 432037. DOI: 10.1073/pnas.74.11.4772. View

19.

Shinozaki K, Okazaki T . T7 gene 6 exonuclease has an RNase H activity. Nucleic Acids Res. 1978; 5(11):4245-61. PMC: 342746. DOI: 10.1093/nar/5.11.4245. View

20.

HOBOM G, Grosschedl R, Lusky M, Scherer G, Schwarz E, Kossel H . Functional analysis of the replicator structure of lambdoid bacteriophage DNAs. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 1:165-78. DOI: 10.1101/sqb.1979.043.01.023. View