Routine Determination of Plasma Catecholamines Using Reversed-phase, Ion-pair High-performance Liquid Chromatography with Electrochemical Detection

Overview

Journal J Chromatogr

Date 1982 Aug 13

PMID 7119064

Citations 10

Authors

C L Davies

S G Molyneux

Affiliations

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Abstract

A procedure is described for the determination of plasma catecholamines using reversed-phase, ion-pair high-performance liquid chromatography coupled with electrochemical detection. Optimisation of chromatographic conditions with respect to detector performance and adherence to procedures and precautions described, render the method applicable to both neurochemical research and routine clinical analysis. The limit of quantitative detection of the method was found to be approximately 30 pg per injection for individual catecholamines. A single chromatographic run, providing adequate resolution of each component, could be completed in approximately 12 min.

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