Sex-limited Genetic Variation in a Mouse Salivary Protein

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1982 Jun 1

PMID 7115283

Citations 5

Authors

R C Karn

S R Dlouhy

K R Springer

J P Hjorth

J T Nielsen

Affiliations

Soon will be listed here.

Abstract

This report describes a gene which influences the electrophoretic mobility of a protein in the salivas of adult mice. Three categories of phenotype have been observed: the two single-banded types, F (Fast) and S (Slow), and the two-banded type, SF (Slow-Fast), with the two bands represented in varying proportions. All females, regardless of age or strain, and all males before puberty show only the F phenotype. Males of the BALB/c and C57BL/6J strains show the F phenotype throughout puberty and adult life, whereas males of the C3H/St and C57BL/KsJ strains show the SF phenotype in puberty and the S phenotype in adult life. We have designated this variation the sex-limited saliva pattern (Ssp). The results from genetic crosses indicate that the variation among the strains is determined by an autosomal locus, Ssp, with two alleles, SspS and SspF, where SspS is dominant to SspF. Testosterone treatment can accelerate the acquisition of the S type in males of the strains C3H/St and C57BL/KsJ and also induces that phenotype in C3H/St females and C57BL/6J males. Thus it appears that the observed strain-specific differences reflect a genetic variation in androgen levels and/or androgen sensitivity rather than variation in a structural gene.

Citing Articles

Positive selection shaped the convergent evolution of independently expanded kallikrein subfamilies expressed in mouse and rat saliva proteomes.

Karn R, Laukaitis C PLoS One. 2011; 6(6):e20979.

PMID: 21695125 PMC: 3114847. DOI: 10.1371/journal.pone.0020979.

The tissue source and cellular control of the apparent size of androgen binding protein (Abp), a mouse salivary protein whose electrophoretic mobility is under the control of sex-limited saliva pattern (Ssp).

Dlouhy S, Karn R Biochem Genet. 1983; 21(11-12):1057-70.

PMID: 6686932 DOI: 10.1007/BF00488459.

Multiple gene action determining a mouse salivary protein phenotype: identification of the structural gene for androgen binding protein (Abp).

Dlouhy S, Karn R Biochem Genet. 1984; 22(7-8):657-67.

PMID: 6541906 DOI: 10.1007/BF00485851.

Production of an antibody to mouse salivary androgen binding protein (ABP) and its use in identifying a prostate protein produced by a gene distinct from Abp.

Dlouhy S, Nichols W, Karn R Biochem Genet. 1986; 24(9-10):743-63.

PMID: 3778428 DOI: 10.1007/BF00499007.

Susceptibility to db gene and streptozotocin-induced diabetes in C57BL mice: control by gender-associated, MHC-unlinked traits.

Leiter E, Le P, Coleman D Immunogenetics. 1987; 26(1-2):6-13.

PMID: 3610255 DOI: 10.1007/BF00345448.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Parker K, Roos M, Shreffler D . Structural characterization of the murine fourth component of complement and sex-limited protein and their precursors: evidence for two loci in the S region of the H-2 complex. Proc Natl Acad Sci U S A. 1979; 76(11):5853-7. PMC: 411750. DOI: 10.1073/pnas.76.11.5853. View

Passmore H, Shreffler D . A sex-limited serum protein variant in the mouse: inheritance and association with the H-2 region. Biochem Genet. 1970; 4(3):351-65. DOI: 10.1007/BF00485752. View

Karn R, Friedman R, MERRITT A . Human salivary proline-rich (Pr) proteins: a posttranslational derivation of the phenotypes. Biochem Genet. 1979; 17(11-12):1061-77. DOI: 10.1007/BF00504345. View

Karn R, Shulkin J, MERRITT A, Newell R . Evidence for post-transcriptional modification of human salivary amylase (amyl) isozymes. Biochem Genet. 1973; 10(4):341-50. DOI: 10.1007/BF00485989. View

Bray G, York D . Genetically transmitted obesity in rodents. Physiol Rev. 1971; 51(3):598-646. DOI: 10.1152/physrev.1971.51.3.598. View

Karn R . The comparative biochemistry, physiology, and genetics of animal alpha-amylases. Adv Comp Physiol Biochem. 1978; 7:1-103. DOI: 10.1016/b978-0-12-011507-5.50007-0. View

Karn R . Sex-limited effects of the expression of the db gene in mice during puberty. Biochem Genet. 1981; 19(3-4):355-71. DOI: 10.1007/BF00504280. View

Paigen K, Swank R, Tomino S, GANSCHOW R . The molecular genetics of mammalian glucuronidase. J Cell Physiol. 1975; 85(2 Pt 2 Suppl 1):379-92. DOI: 10.1002/jcp.1040850406. View

10.

Azen E . Genetic protein polymorphisms in human saliva: an interpretive review. Biochem Genet. 1978; 16(1-2):79-99. DOI: 10.1007/BF00484386. View

11.

Szoka P, Paigen K . Regulation of mouse major urinary protein production by the Mup-A gene. Genetics. 1978; 90(3):597-612. PMC: 1213907. DOI: 10.1093/genetics/90.3.597. View

12.

Owerbach D, Hjorth J . Inheritance of a parotid secretory protein in mice and its use in determining salivary amylase quantitative variants. Genetics. 1980; 95(1):129-41. PMC: 1214211. DOI: 10.1093/genetics/95.1.129. View

13.

Toole J, Hastie N, Held W . An abundant androgen-regulated mRNA in the mouse kidney. Cell. 1979; 17(2):441-8. DOI: 10.1016/0092-8674(79)90170-3. View

14.

Azen E, Denniston C . Genetic polymorphism of human salivary proline-rich proteins: further genetic analysis. Biochem Genet. 1974; 12(2):109-20. DOI: 10.1007/BF00487820. View

15.

Berger M, de Turckheim M, Veyssiere G, Jean C . Developmental patterns of plasma and testicular testosterone in mice from birth to adulthood. Acta Endocrinol (Copenh). 1978; 89(4):780-8. DOI: 10.1530/acta.0.0890780. View

16.

MERRITT A, Karn R . The human alpha-amylases. Adv Hum Genet. 1977; 8:135-234. View

17.

Studier F . Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973; 79(2):237-48. DOI: 10.1016/0022-2836(73)90003-x. View

18.

Hjorth J, Lusis A, Nielsen J . Multiple structural genes for mouse amylase. Biochem Genet. 1980; 18(3-4):281-302. DOI: 10.1007/BF00484242. View

19.

Nielsen J . Variation in the number of genes coding for salivary amylase in the bank vole, clethrionomys glareola. Genetics. 1977; 85(1):155-69. PMC: 1213614. DOI: 10.1093/genetics/85.1.155. View